HPLC测定不同产地滇牡丹中没食子酸和丹皮酚含量

张德全; 马阿丽; 杨永寿; 黄传会; 周浓

doi:10.11653/syfj2014050057

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HPLC测定不同产地滇牡丹中没食子酸和丹皮酚含量

更新时间：2024-01-04

- HPLC测定不同产地滇牡丹中没食子酸和丹皮酚含量
- Content Determination of Trihydroxybenzoic Acid and Paeonol in from Different Producing Area by HPLC
- 中国实验方剂学杂志 2014年20卷第5期页码：57-60
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（31200180）
- DOI：10.11653/syfj2014050057
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
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张德全, 马阿丽, 杨永寿, 等. HPLC测定不同产地滇牡丹中没食子酸和丹皮酚含量[J]. 中国实验方剂学杂志, 2014,20(5):57-60.

ZHANG De-quan, MA A-li, YANG Yong-shou, et al. Content Determination of Trihydroxybenzoic Acid and Paeonol in from Different Producing Area by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 57-60.
张德全, 马阿丽, 杨永寿, 等. HPLC测定不同产地滇牡丹中没食子酸和丹皮酚含量[J]. 中国实验方剂学杂志, 2014,20(5):57-60. DOI： 10.11653/syfj2014050057.

ZHANG De-quan, MA A-li, YANG Yong-shou, et al. Content Determination of Trihydroxybenzoic Acid and Paeonol in from Different Producing Area by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 57-60. DOI： 10.11653/syfj2014050057.

摘要

目的：建立滇牡丹中没食子酸和丹皮酚含量测定方法，并比较滇牡丹不同产地及其不同部位中没食子酸和丹皮酚的含量。方法：采用ZORBOX SB-C18（4.6 mm×250 mm，5 μm）色谱柱，以乙腈-0.1%甲酸溶液为流动相，梯度洗脱，流速1.0 mL·min-1，检测波长275 nm，柱温30 ℃。结果：没食子酸和丹皮酚在0.204 0~2.040 μg和0.204 0~2.040 μg呈良好的线性关系，平均加样回收率（n=6）为101.81%，97.80%。结论：该方法可用于滇牡丹药材的质量控制；不同产地滇牡丹中没食子酸和丹皮酚的含量差异较大。

Abstract

Objective: To establish determined method of the content of trihydroxybenzoic acid and paeonol in Paeonia delavayi

and compare the content in samples from different areas and samples from different parts. Method: The samples were separated by a ZORBOX SB-C18 (4.6 mm×250 mm

5 μm) using acetonitrile-0.1% methane acid with water gradient system as mobile phase. The flow rate was 1.0 mL·min-1

and the detection wavelength for fingerprinting was 275 nm with 30 ℃ for column temperature. Result: There were well linear relationships for trihydroxybenzoic acid and paeonol in range of 0.204 0-2.040 μg and 0.204 0-2.040 μg. And average recovery rates for them (n=6) were 101.81% and 97.80%. Conclusion: The method could be used for quality control to medicinal materials of P. delavayi. Contents of trihydroxybenzoic acid and paeonol were significantly different for medicinal materials collected from different areas.

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