Objective: To analyze and determine the stability of icariside and its factors affecting in different conditions. Method: On the basis of ultraviolet-visible spectrum under 200-600 nm region scan

determine the factors affecting the chemical structure of icariside in different conditions. Result: Icariside was stable in water

incruding slightly acidic

basic and neutral conditions.When it existed in aqueous phase under pH<2 or pH>10 conditions

the chemical structure of icariside was changed. Furthermore

its aqueous phase under 100 ℃ for a long time was remain stable. In the processes of extracting and using icariside

metal ion such as Cu2+

Fe3+and Al3+ should be wiped off

as they were able to chelate with icariside

and could induce oxidation as well. Conclusion: Using ultraviolet-visible spectrum can primary observation the factors affecting the stability of icariside in different conditions

and providing important information on its extraction

preparation and derivatives preparation.