Objective: To further investigate the chemical constituents from the petroleum ether and chloroform fractions of ethanol extract from Spiraea pubescens. Method: The compounds were isolated by silica gel chromatography

ODS chromatography

and preparative HPLC

and their structures were elucidated on the basis of chemical and spectroscopic methods

including MS

1D and 2D NMR spectral techniques. Result: Nine compounds were isolated from the petroleum ether and chloroform fractions of ethanol extract from S. pubescens

and were identified as ursolic acid (1)

ursolic aldehyde (2)

ursonic acid (3)

germanicol (4)

α-amyrenol (5)

erythrodiol (6)

betulinic acid (7)

oleanolic acid (8)

and betulonic acid (9). Conclusion: This is the first report of the isolation of 2-6 and 8-9 from Spiraea genus

and 1

7 from this plant.