构橘叶挥发油的化学成分及活性研究

黄国华; 张大帅; 宋鑫明; 孙丽君; 宋煌旺; 李愈娴; 张琼玉; 周瑾

doi:10.11653/syfj2014050097

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构橘叶挥发油的化学成分及活性研究

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- 构橘叶挥发油的化学成分及活性研究
- Chemical Components, Cytotoxic and Antimicrobial Activity of Essential Oil of Leaves of
- 中国实验方剂学杂志 2014年20卷第5期页码：97-101
- 作者机构：
- 作者简介：
- 基金信息：
  
  新世纪优秀人才项目（NCET-08-0656）;海南省中药现代化基金项目（2011ZY010）
- DOI：10.11653/syfj2014050097
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
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黄国华, 张大帅, 宋鑫明, 等. 构橘叶挥发油的化学成分及活性研究[J]. 中国实验方剂学杂志, 2014,20(5):97-101.

HUANG Guo-hua, ZHANG Da-shuai, SONG Xin-ming, et al. Chemical Components, Cytotoxic and Antimicrobial Activity of Essential Oil of Leaves of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 97-101.
黄国华, 张大帅, 宋鑫明, 等. 构橘叶挥发油的化学成分及活性研究[J]. 中国实验方剂学杂志, 2014,20(5):97-101. DOI： 10.11653/syfj2014050097.

HUANG Guo-hua, ZHANG Da-shuai, SONG Xin-ming, et al. Chemical Components, Cytotoxic and Antimicrobial Activity of Essential Oil of Leaves of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 97-101. DOI： 10.11653/syfj2014050097.

摘要

目的：提取构橘叶挥发性成分，进行抗菌抗肿瘤活性测试，为进一步研究开发构橘提供实验依据。方法：利用水蒸气蒸馏法制备构橘叶挥发油，计算收率，并结合GC-MC分析化学成分；用纸片琼脂扩散法和MTT比色法分别研究构橘叶挥发油抗菌和抗肿瘤活性。结果：水蒸气蒸馏法提取构橘叶挥发油收率为0.78%，得到65个化合物，鉴定出51个，占总成分的91.25%；构橘叶挥发油对枯草杆菌具有较好的抑菌活性，对白血病细胞（K-562）、肝癌细胞（BEL-7402）具有一定的抑制活性，IC50分别为31.53和36.84 g·mL-1。结论：构橘叶挥发油的主要成分为芳樟醇氧化物（11.93%）、蓝桉醇（10.18%）、喇叭茶萜醇（8.92%）等。抗菌实验结果表明构橘叶挥发油对枯草杆菌具有较好的抗菌活性。抗肿瘤活性实验结果显示构橘叶挥发油对白血病细胞K-562和肝癌细胞BEL-7402细胞具有抑制增殖的活性。

Abstract

Objective: To study the chemical constituents and bioactivity of essential oil from Poncirus trifoliata. Method: The essential oil was extracted by hydrodistillation and separated by capillary GC. The chemical constituents were determined by normalization and were identified by MS. Result: 65 chromatographic peaks were detected and 51 compounds were identified

which were 91.25% of the total essential oil. The cytotoxicity of the essential oil against human cancer cell lines BEL-7402

K-562

SGC-7901 and SPCA-1 was investigated in vitro. Conclusion: The major components of the essential oil were linalool oxide (11.93%)

(-)-globulol (10.18%) and Ledol (8.92%). The oil showed moderate activity against K-562 and BEL-7402 human tumor cell lines with IC50 values less than 40 μg/ml. The oil was also found to possess antimicrobial activity against Bacillus subtilis.

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