Objective: To investigate the metabolic profile of stagnant heat syndrome in rats and the intervention effects of Qinghuoling. Method: There were control

model and Qinghuoling groups.Model and Qinghuoling groups were treated with special feed and ip atropine (0.25 mg·kg-1)

twice a day

continues modeling for 7 days

from the 4 th day of modeling

ig Qinghuoling(2.64 g·kg-1)

once a day.Proton nuclear magnetic resonance(1H-NMR) technique was used to analyze the rat serum and urine metabolic profiles. Multivariate data analysis methods such as orthogonal partial least squares discriminant analysis (OPLS-DA) and principal components analysis (PCA)

and univariate statistical analysis methods were used to analyze the metabonome difference between the control and model group samples

to identify the corresponding metabolic biomarkers and to study the intervention effect of Qinghuoling. The mechanism of action of Qinghuoling was illustrated through the selected biomarkers. Result: Compared with the control group

rectal temperature

water intake

and food intake of model group has a significant change The metabolic phenotype of rats in model group greatly changed

Lipid metabolism

glucose uptake and metabolism

intestinal flora have been affected in different degrees and Qinghuoling had efficacious intervention. Six and sevenpotential biomarkers were selected in rat serum and urine

respectively. Conclusion: Qinghuoling can effectively improve the disturbance of lipids and glucose metabolismand modulate the gut microflora environment in rats.