Objective: To explore the effect of Chinese herbal compound Tangbikang(TBK) on the expressions of nerve growth factor (NGF) protein and NGF mRNA of diabetic rats' sciatic nerve. Method: Streptozotocin(STZ) and feeding high fat forage induced diabetic rats were randomly divided into diabetic model group

Mecobalamin group

TBK high dose group

TBK middle dose group

TBK low dose group and SD normal male rats (n=10). Normal control group and model group were daily gavaged with distilled water

TBK groups were given TBK with different dose(4.175

8.35

16.70 g·kg-1). Mecobalamin (1.97 mg·kg-1) was used as the control medicine. Rats' unilateral sciatic nerve were taken out at the 16th week

immunohistochemical method was used to detect the expressions of NGF protein of sciatic nerve

and the expressions of NGF mRNA were detected by semi-quantitative RT-PCR. Result: Compared with normal group

the expressions of NGF protein and NGF mRNA of model group were significantly decreased (P<0.01)

Compared with the model group

high and middle dose TBK group significantly increased the expressions of NGF protein and NGF mRNA (P<0.01) and low dose TBK group significantly increased the expressions of NGF mRNA (P<0.05). Conclusion: Chinese herbal compound TBK could promote the expressions of NGF and NGF mRNA of diabetic peripheral neuropathy rats' sciatic nerve.