6味山姜属中药对胃实寒证大鼠胃组织PDE,cAMP,cGMP,cAMP/cGMP的影响

黄燕琼; 秦华珍; 柳俊辉; 王晓倩; 刘磊; 余腾飞; 刘颖; 李文强; 谭喜梅

doi:10.11653/syfj2014050161

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6味山姜属中药对胃实寒证大鼠胃组织PDE,cAMP,cGMP,cAMP/cGMP的影响

更新时间：2024-01-04

- 6味山姜属中药对胃实寒证大鼠胃组织PDE,cAMP,cGMP,cAMP/cGMP的影响
- Effect of Six Alpinia Roxb on PDE, cAMP, cGMP, cAMP/cGMP of Gastric Tissue of Stomach in Excessive Cold Syndrome Rats
- 中国实验方剂学杂志 2014年20卷第5期页码：161-165
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西教育厅课题（201012MS144）;广西中医学院课题（P2009043）
- DOI：10.11653/syfj2014050161
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
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黄燕琼, 秦华珍, 柳俊辉, 等. 6味山姜属中药对胃实寒证大鼠胃组织PDE,cAMP,cGMP,cAMP/cGMP的影响[J]. 中国实验方剂学杂志, 2014,20(5):161-165.

HUANG Yan-qiong, QIN Hua-zhen, LIU Jun-hui, et al. Effect of Six Alpinia Roxb on PDE, cAMP, cGMP, cAMP/cGMP of Gastric Tissue of Stomach in Excessive Cold Syndrome Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 161-165.
黄燕琼, 秦华珍, 柳俊辉, 等. 6味山姜属中药对胃实寒证大鼠胃组织PDE,cAMP,cGMP,cAMP/cGMP的影响[J]. 中国实验方剂学杂志, 2014,20(5):161-165. DOI： 10.11653/syfj2014050161.

HUANG Yan-qiong, QIN Hua-zhen, LIU Jun-hui, et al. Effect of Six Alpinia Roxb on PDE, cAMP, cGMP, cAMP/cGMP of Gastric Tissue of Stomach in Excessive Cold Syndrome Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 161-165. DOI： 10.11653/syfj2014050161.

摘要

目的：研究高良姜、草豆蔻、红豆蔻、大高良姜、建砂仁、益智6味山姜属中药对胃实寒证模型大鼠胃组织磷酸二酯酶（PDE）、环磷酸腺苷（cAMP）、环磷酸鸟苷（cGMP）、cAMP/cGMP的影响，揭示6味山姜属中药所含挥发油与温中散寒功效的相关性。方法：实验分2批进行，第1批取SD雄性大鼠160只分为16个组：模型Ⅰ组，模型Ⅱ组，阳性对照组，高良姜、草豆蔻、红豆蔻3味药物各个挥发油高、低剂量组、去挥发油水液高、低剂量组；第2批除了观察药为大高良姜、建砂仁、益智挥发油高、低剂量组、去挥发油水液高、低剂量组外，其余分组均同第1批。采用灌服2~3 ℃寒凉药（石膏、知母、黄柏、龙胆草，给药剂量为36 g·kg-1，2次/d）加冷冻的方法制造大鼠胃实寒证模型；分别予以6味药物挥发油、去挥发油水液，低剂量组给药剂量为20 g·kg-1，高剂量组给药剂量为60 g·kg-1，阳性对照药选用附子水煎液，剂量为8 g·kg-1；每隔12 h给药1次，连续给药3次。测定大鼠胃组织PDE，cAMP，cGMP含量，计算cAMP/cGMP。结果：①对磷酸二酯酶（PDE）的影响：与正常组相比，第1，2批模型组胃组织中PDE明显升高（P<0.05或P<0.01），分别为32.27，52.80 nmol·L-1。高良姜、草豆蔻、红豆蔻、大高良姜、益智的挥发油高、低剂量组对于模型组大鼠胃中PDE有显著的降低作用（P<0.05或P<0.01），大高良姜去挥发油高剂量组对其也有一定的降低作用，但无显著性差异；②对cAMP，cGMP及cAMP/cGMP的影响：与空白组比较，第1，2批模型组胃组织中cAMP的浓度明显降低（P<0.05），分别为0.68，0.81 nmol·L-1。6味中药挥发油高、低剂量组与模型组相比，cAMP浓度均明显升高（P<0.05或P<0.01），cGMP无明显变化，cAMP/cGMP增大（P<0.05或P<0.01）；高良姜、草豆蔻、建砂仁去挥发油高剂量组对cAMP浓度也有一定的影响，但无显著性差异。结论：6味中药所含挥发油可升高胃组织中cAMP的含量、cAMP/cGMP及降低PDE的含量，其中部分中药的去挥发油高剂量组也有相似的影响，揭示了6味山姜属中药所含的挥发油与温中散寒功效有密切的相关性，且发现此6味药的去挥发油提取液对部分检测指标也有良好的改善作用，揭示其功效也与其所含的其他成分有关。

Abstract

Objective: To study the effect of the six Alpinia Roxbe on phosphodiesterase(PDE)

cyclic Adenosine monophosphate(cAMP)

cyclic guanosine monophosphate(cGMP)

cAMP/cGMP of the gastric tissue of stomachin excessive cold syndrome rats

and reveal the correlation between the six kinds volatile oil of Alpinia Roxb. and the function of warming middle energizer and dispersing cold. Method: By giving cold-cool nature Chinese medicines and freezing method

stomach excessive cold syndrome rat models was established. The high and low dose of the volatile oil and the water extract removed volatile oil of the six Chinese medicine were given to rat

the positive control drug was given water extract of aconite. PDE

cAMP and cGMP

and calculated cAMP/cGMP ratio were determined. Result: ①The high and low dose groups of the volatile oil of the six Chinese medicine significantly reduced the concentration of stomach PDE in model rats(P<0.05 or P<0.01)

high dose group of water extract removed volatile oil of Alpinia galanga also had a certain reduction effect

but no significant difference.②The high and low dose groups of the volatile oil of the six Chinese medicine compared with model group

cAMP concentrations were significantly increased (P<0.05 or P<0.01)

cGMP no significant changes

cAMP/cGMP ratio increased (P<0.05 or P<0.01);the high dose group of water extract removed volatile oil of galangal

Alpinia katsumadai

Villous amomum fruit also had some influence on the concentration of cAMP

but no significant difference. Conclusion: The volatile oil of the six Alpinia Roxb may elevate gastric tissue cAMP levels

cAMP/cGMP ratio

reduce the PDE content

some Chinese medicine without volatile oil at high dose also had a similar effect. The volatile oil of the six Alpinia Roxb was related the function of warming middle energizer and dispersing cold

and water extract removed volatile oil of the six Chinese medicine also had some good improvement

revealing their effectiveness is also associated with other ingredients contained.

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