Objective: To study the inhibitory effect of compound Yulangsan polysaccharide (CYLSP) on duck hepatitis B virus (DHBV) persistent infection. Method: One-day old Guangxi brown spotted ducks infected with hepatitis B virus (DHBV) were used as the DHBV infected animal model. Fifty positive ducks were detected by PCR at one week after the infection of DHBV

then randomly divided into 5 groups

each group of 10

only CYLSP high

medium and low dose group (10

5

2.5 g·kg-1) and lamivudine (0.05 g·kg-1) group and model group

the daily morning lavage l time for 14 d. Alanine aminotransferase(ALT)

aspartate aminotransferase(AST) in serum were detected before treatment (T0)

7 days (T7)

14 days (T14) after treatment

and 3 days after stopping treatment. At 3 days after stopping treatment

the livers were collected

homogenate was prepared to detect superoxide dismutase(SOD)

glutathione peroxidase(GSH-Px) activity and reduced glutathion(GSH)

malondialdehyde(MDA) levels

HE staining was used to observe the pathological change. Result: Compared with T0 and model group

the serum levels of ALT and AST were decreased significantly and 3 days after stopping treatment

CYLSP high dose group and lamivudine group still showed a protection effect (P<0.05 or P<0.05) SOD

GSH-Px activity and the content of GSH

MDA.CYLSP in high

middle dose group and lamivudine group showed a protection action. In CYLSP low dose group

no change of SOD

GSH-Px activity and GSH

MDA content was found

HE staining showed CYLSP significantly reduced the duck liver histopathologic damage degree. Conclusion: The compound Yulangsan polysaccharide has a protection action on duck liver injury induced by duck hepatitis B virus