姜黄素眼用脂质体的制备及晶状体抗氧化作用考察

倪斌; 李翔; 罗云; 张婧; 廖正根; 刘婧; 林艳; 崔汉峰

doi:10.11653/syfj2014060005

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姜黄素眼用脂质体的制备及晶状体抗氧化作用考察

更新时间：2024-01-04

- 姜黄素眼用脂质体的制备及晶状体抗氧化作用考察
- Preparation of Curcumin-Loaded Ophthalmic Liposomes and Investigation of Its Lenses Antioxidant Efficacy
- 中国实验方剂学杂志 2014年20卷第6期页码：5-9
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81202927）;江西省研究生创新专项基金项目（YC2012-S106）;江西省教育厅青年基金项目（GJJ12535，GJJ12536）;江西省卫生厅中医药科研计划（普通）课题（2012A160，2012A157）;江西省青年科学基金项目（20132BAB215022）
- DOI：10.11653/syfj2014060005
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
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倪斌, 李翔, 罗云, 等. 姜黄素眼用脂质体的制备及晶状体抗氧化作用考察[J]. 中国实验方剂学杂志, 2014,20(6):5-9.

NI Bin, LI Xiang, LUO Yun, et al. Preparation of Curcumin-Loaded Ophthalmic Liposomes and Investigation of Its Lenses Antioxidant Efficacy[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 5-9.
倪斌, 李翔, 罗云, 等. 姜黄素眼用脂质体的制备及晶状体抗氧化作用考察[J]. 中国实验方剂学杂志, 2014,20(6):5-9. DOI： 10.11653/syfj2014060005.

NI Bin, LI Xiang, LUO Yun, et al. Preparation of Curcumin-Loaded Ophthalmic Liposomes and Investigation of Its Lenses Antioxidant Efficacy[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 5-9. DOI： 10.11653/syfj2014060005.

摘要

目的：制备N-三甲基壳聚糖（TMC）包覆姜黄素眼用脂质体并考察其抑制大鼠硒性白内障晶状体氧化作用。方法：采用乙醇注入法制备姜黄素脂质体（Cur-TCL），通过星点设计-效应面法优化脂质体处方工艺；采用过膜法、透射电镜、粒径电位测定仪测定Cur-TCL的理化性质；建立大鼠硒性白内障模型，考察Cur-TCL对白内障形成过程中晶状体氧化的抑制作用。结果：最佳处方为姜黄素1.41 mg，磷脂质量分数4%，胆固醇-磷脂（1：5.44）。经0.5%TMC溶液包覆后脂质体呈球形，粒径增大，电位显著提高，药物包封率基本不变。与未包覆脂质体相比，Cur-TCL组大鼠晶状体中SOD活力提高30.3%，MDA摩尔浓度减少52.4%，GSH含量增加1.29倍。结论：Cur-TCL可显著抑制硒性白内障导致的晶状体氧化过程，为该制剂治疗白内障提供参考。

Abstract

Objective: To prepare N-trimethyl chitosan(TMC)-coated liposomes loaded with curcumin (Cur-TCL) and investigate its in vivo antioxidant effect on selenite-induced cataract lenses. Method: Cur-TCL were prepared by ethanol injection method and formulation was optimized by central composite design-response surface methodology;Membrane filter

transmission electron microscope

dynamic light scattering and electrophoretic light scattering were employed to determined physicochemical parameters of Cur-TCL;Selenite cataract model in rats was established

inhibition of Cur-TCL on oxidation process in selenite cataract formation was investigated. Result: Optimal formulation was as following:phospholipids concentration 4%

cholesterol-phospholipid(1:5.44)

curcumin 1.41 mg and the concentration of TMC solution 0.5%.Cur-TCL was spherical-shaped with increasing of mean particle sizes

signigicantly improving of Zeta potential and drug encapsulation efficiency basically unchanged(92.5±0.35)% after coating liposomes with TMC.Compared with uncoated liposomes

SOD activity was elevated by 30.3%

molar concentration of MDA decreased by 52.4% and the content of GSH increased 1.29-fold. Conclusion: Cur-TCL could significantly delay lenses oxidation process of selenite cataract.This work was useful for further research on application of Cur-TCL for cataract therapy.

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