Objective: To optimize purification technology of total flavonoids from Callicarpa nudiflora by polyamide resin. Method: With the content of total flavonoids as index

adsorption and elution behavior of polyamide resin for total flavonoids was investigated by stastic-dynamic adsorption-elution test

single factor tests were adopted to optimize purification technology for total flavonoids taking saturated adsorption

pH

elution volume and other parameters. Result: Optimum purification technology parameters were as follows:the concentration of sample solution 0.073 g·mL-1

sample volume of 2 BV

pH of sample solution 3.0

eluted impurities with 3 BV water

then eluted with 3 BV of 80% ethanol;Purity of total flavonoids was up to 42.3% from 5.6%

average retention rate was 89.6%(RSD 3.56%). Conclusion: Purification effect of total flavonoids from C. nudiflora by polyamide resin was feasible

and this study could provide experimental basis for development of new formulations from C. nudiflora.