正交试验优化“天仙红”桃果肉中花青素的提取工艺

周静; 王富荣; 黄华鹏; 何华平; 徐凌云

doi:10.11653/syfj2014060030

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正交试验优化“天仙红”桃果肉中花青素的提取工艺

更新时间：2024-01-04

- 正交试验优化“天仙红”桃果肉中花青素的提取工艺
- Optimization of Extraction Technology for Anthocyanins from ‘Tianxianhong’ Peach Flesh by Orthogonal Test
- 中国实验方剂学杂志 2014年20卷第6期页码：30-32
- 作者机构：
- 作者简介：
- 基金信息：
  
  湖北省农业科技创新项目（2011-620-005-003-04）
- DOI：10.11653/syfj2014060030
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
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周静, 王富荣, 黄华鹏, 等. 正交试验优化“天仙红”桃果肉中花青素的提取工艺[J]. 中国实验方剂学杂志, 2014,20(6):30-32.

ZHOU Jing, WANG Fu-rong, HUANG Hua-peng, et al. Optimization of Extraction Technology for Anthocyanins from ‘Tianxianhong’ Peach Flesh by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 30-32.
周静, 王富荣, 黄华鹏, 等. 正交试验优化“天仙红”桃果肉中花青素的提取工艺[J]. 中国实验方剂学杂志, 2014,20(6):30-32. DOI： 10.11653/syfj2014060030.

ZHOU Jing, WANG Fu-rong, HUANG Hua-peng, et al. Optimization of Extraction Technology for Anthocyanins from ‘Tianxianhong’ Peach Flesh by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 30-32. DOI： 10.11653/syfj2014060030.

摘要

目的：优选“天仙红”桃果肉中花青素的提取工艺。方法：以花青素提取量为指标，在单因素试验基础上，通过正交试验考察料液比、提取液pH、乙醇体积分数、提取时间对花青素提取工艺的影响。采用HPLC测定花青素含量，色谱条件为流动相4%磷酸-乙腈（85：15，pH 2.0），检测波长520 nm。结果：各因素对花青素提取量的影响顺序为提取液pH >料液比 >乙醇体积分数 >提取时间。最佳提取工艺为加10倍量20%乙醇溶液（pH 2.5）于40 ℃提取3 h；花青素提取量达55.8 μg·g-1。结论：优选的提取工艺简单可行，为“天仙红”桃的开发利用提供实验依据。

Abstract

Objective: To optimize extraction technology of anthocyanins from ‘tianxianhong’ peach flesh. Method: With yield of anyhocyanins as index

based on single factor tests

orthogonal test was adopted to optimize extraction technology by taking ethanol concentration

pH of extract

extraction time and solid-liquid ratio as factors.HPLC was used to determine the content of anyhocyanins with mobile phase of 4% phosphoric acid-acetonitrile(85:15

pH 2.0) and detection wavelength of 520 nm. Result: The order of factors influencing yield of anthocyanins was pH of extract >solid-liquid ratio >ethanol concentration >extraction time.Optimum extraction technology was as following:extracted 3 h with 10 times the amount of 20% ethanol(pH 2.5) at 40 ℃;Yield of anthocyanins was up to 55.8 μg·g-1. Conclusion: This optimized extraction process was simple and feasible

it would provide experimental basis for further development and utilization of ‘tianxianhong’ peach.

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