Objective: To investigate the chemical compositions of the Trillium tschonoskii. Method: The compounds were isolated and purified from the plant with chromatography techniques and the structures were identified onthe basis of spectral analyses and physicochemical properties. Result: Ten compounds were isolated from T. tschonoskii and their structures were identified as pennogenin-3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside(1)

pennogenin-3-O-α-L-rhamno-pyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside (2)

pennogenin-3-O-α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside (3)

stigmasterol (4)

β-sitosterol(5)

daucosterol(6)

astragalin(7)

stigmasterol-3-O-β-D-glucopyranoside-６'-hexacosanoate(8)

stigmasterol-3-O-β-D-glucopyranoside(9)

ursolic acid(10). Conclusion: Compounds 4

8

9

10 were isolated from Trillium for the first time.