HPLC测定不同品种金银花及叶中木犀草苷含量

赵金娟; 管仁伟; 路俊仙; 王萌; 杜肖璇; 林慧彬; 林建强

doi:10.11653/syfj2014060103

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HPLC测定不同品种金银花及叶中木犀草苷含量

更新时间：2024-01-04

- HPLC测定不同品种金银花及叶中木犀草苷含量
- Determination of Content of Galuteolin in Different Varieties of Flos and Folium by HPLC
- 中国实验方剂学杂志 2014年20卷第6期页码：103-106
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家“十二五”支撑计划项目（2011BAI06B01）
- DOI：10.11653/syfj2014060103
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
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赵金娟, 管仁伟, 路俊仙, 等. HPLC测定不同品种金银花及叶中木犀草苷含量[J]. 中国实验方剂学杂志, 2014,20(6):103-106.

ZHAO Jin-juan, GUAN Ren-wei, LU Jun-xian, et al. Determination of Content of Galuteolin in Different Varieties of Flos and Folium by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 103-106.
赵金娟, 管仁伟, 路俊仙, 等. HPLC测定不同品种金银花及叶中木犀草苷含量[J]. 中国实验方剂学杂志, 2014,20(6):103-106. DOI： 10.11653/syfj2014060103.

ZHAO Jin-juan, GUAN Ren-wei, LU Jun-xian, et al. Determination of Content of Galuteolin in Different Varieties of Flos and Folium by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 103-106. DOI： 10.11653/syfj2014060103.

摘要

目的：比较不同品种之间金银花及叶中黄酮类成分木犀草苷含量的差异。方法：采用Diamonsil-C18色谱柱（150 mm×4.6 mm，5 μm），乙腈-0.5%冰醋酸溶液为流动相梯度洗脱，流速1.0 mL·min-1，柱温35 ℃，检测波长350 nm。结果：木犀草苷在0.015 6~0.5 g·L-1呈良好线性关系，平均回收率为96.88%。结论：1~10号金银花样品中木犀草苷的含量在0.08%~0.22%之间，均高于《中国药典》规定的0.050%，其中亚特良种金银花（意大利种质）及九丰一号含量较高。红金银花中木犀草苷含量为0.11%，符合《中国药典》该指标要求。金银花叶中木犀草苷的含量分别为0.75%，0.76%，明显高于花中木犀草苷的含量。

Abstract

Objective: To compare differences of the content of galuteolin between different varieties of Lonicerae japonicae Flos and L. Japonicae Folium. Method: The separation was performed on a Diamonsil-C18 column(150 mm×4.6 mm

5 μm)

acetonitrile-0.5% acetic acid was used as the mobile phase with gradient elution

the flowrate was 1.0 mL·min-1

column temperature was 35 ℃

the detection wavelength was at 350 nm. Result: The linear range (n=6)was 0.015 625-0.5 g·L-1 (R2=1) respectively. The averagerecoveries of galuteolin was 96.88% respectively. Conclusion: The content of galuteolin in from 1 to 10 sample is between 0.08% and 0.22%

which is higher than 0.050% of requirement of ChP.The content of galuteolin in ya te improved variety L. japonicae Flos (introduced from Italy)and jiu feng yi hao is higher than others samples. The content of galuteolin in L. japonica var.chinensis is 0.11%

which meets the medicinal requirements. The content of galuteolin in L. japonicae Folium is between 0.75% and 0.76%

which is apparently higher than it in L. japonicae Flos.

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