Objective: To evaluate pharmacokinetic characteristic of capsaicin submicron emulsion in rabbits. Method: HPLC was established to determine the concentration of capsaicin in rabbit plasma with mobile phase of methanol-water-phosphoric acid(68：32：0.1) and detection wavelength at 281 nm.compartment models were analyzed by DASTM ver1.0 software after rabbit ear vein administration of capsaicin submicron emulsion and its aqueous

then calculated pharmacokinetic parameters. Result: Linear correlation was satisfactory when the concentration of capsaicin in rabbit plasma was 0.500-100 mg·L-1(r=0.999 7)

extraction recovery was 96.10%-105.48%.Pharmacokinetics curve was both corresponded to two-compartment model after intravenous injection of capsaicin submicron emulsion and its aqueous

elimination half-life were (48.501 3±4.011 9) and (15.262 5±1.888 2) min

area under curve were (924.09±149.80) and (555.36±57.46) mg·min·L-1. Conclusion: Established HPLC was accurate

sensitive and satisfied with concentration determination of capsaicin in rabbit plasma.Compared with capsaicin solution

prolongation of elimination half-life and elevation of biological availability were all obtained after capsaicin was emulsified with submicron emulsion.