玉郎伞多糖抗鸭乙型肝炎病毒作用

郭雪峰; 罗秀; 何巧玲; 李秀菊; 明建军; 黄仁彬; 张士军

doi:10.11653/syfj2014060175

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玉郎伞多糖抗鸭乙型肝炎病毒作用

更新时间：2024-01-04

- 玉郎伞多糖抗鸭乙型肝炎病毒作用
- Yulangsan Polysaccharide Affects Anti-Duck Hepatitis B Virus in Brown Duck
- 中国实验方剂学杂志 2014年20卷第6期页码：175-178
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西地方特有中药有效成分与机制研究（桂教科研 201202ZD023）;广西地方性高发疾病防治重点实验室开放课题（桂科能 0842009-K4）
- DOI：10.11653/syfj2014060175
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
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郭雪峰, 罗秀, 何巧玲, 等. 玉郎伞多糖抗鸭乙型肝炎病毒作用[J]. 中国实验方剂学杂志, 2014,20(6):175-178.

GUO Xue-feng, LUO Xiu, HE Qiao-ling, et al. Yulangsan Polysaccharide Affects Anti-Duck Hepatitis B Virus in Brown Duck[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 175-178.
郭雪峰, 罗秀, 何巧玲, 等. 玉郎伞多糖抗鸭乙型肝炎病毒作用[J]. 中国实验方剂学杂志, 2014,20(6):175-178. DOI： 10.11653/syfj2014060175.

GUO Xue-feng, LUO Xiu, HE Qiao-ling, et al. Yulangsan Polysaccharide Affects Anti-Duck Hepatitis B Virus in Brown Duck[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 175-178. DOI： 10.11653/syfj2014060175.

摘要

目的：研究玉郎伞多糖（YLSP）抗鸭乙型肝炎病毒（duck hepatitis B virus，DHBV）的作用。方法：取1日龄广西麻鸭60只，在麻鸭颈静脉注射0.2 mL强阳性DHBV DNA鸭血清，13 d后分别从其颈静脉采血0.5 mL，分离血清，用普通PCR法筛选出DHBV感染强阳性鸭。然后，将50只DHBV DNA阳性麻鸭随机分为5组：YLSP高、中、低剂量组（5，2.5，1.25 g·kg-1）；阿昔洛韦阳性对照组（0.1 mg·kg-1）和模型组。所有给药组均与模型组比较，以明确玉郞伞多糖对DHBV DNA的作用，所以未设置正常组。于给药前（T0），给药7 d（T7），14 d（T14）及停药后3 d（P3）采血，用实时荧光定量PCR法检测鸭血清中DHBV DNA的含量。HE染色观察肝损伤程度。结果：与模型组相比，YLSP各剂量组及阳性对照组在用药后血清中DHBV DNA的含量显著降低，停药后，阳性对照组及YLSP中、低剂量组DHBV DNA含量均有反跳现象。HE染色结果显示YLSP能减轻鸭肝组织的病理损伤程度。结论：YLSP对DHBV 具有抑制作用同时对肝损伤具有保护作用。

Abstract

Objective: To investigatewhether Yulangsan Polysaccharide (YLSP) affects the anti-Duck Hepatitis B Virus (anti-DHBV) in brown duck. Method: Sixty one-day-old ducks were selected. After 13 days

positive ducks infected 0.2 mL serums of DHBV DNA were detected by PCR

and were randomly divided into five groups:YLSP high-

middle-

low-dose groups(5

2.5

1.25 g·kg-1)

aciclovir(ACV) group(0.1 mg·kg-1) and model group with 10 brown ducksin every group. The serums were respectively collected before administration

7 and 14 days after administration and 3 day after withdrawal. The DHBV DNA was detected by fluorescence quantitative(FQ)-PCR assay. The extent of liver injury was observed by HE staining. Result: Compared with model group

the serum content of DHBV DNA in ACV group and YLSP 5

2.5

1.25 g·kg-1 groups was significantly decreased. The content of DHBV DNA in ACV group

middle-does group and low-dose groups showed a rebound phenomenon after withdrawal. The results indicatedthat YLSP had significant protective effects against the injury of ducks' liver. Conclusion: YLSP not only has an inhibited effect to DHBV

but also has a protective effect on injury of liver.

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