HP-20型大孔树脂富集纯化南山茶花总黄酮的工艺优选

尚志春; 宋明明; 史丽颖; 于大永; 唐玲; 王永奇

doi:10.11653/zgsyfjxzz2013070011

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HP-20型大孔树脂富集纯化南山茶花总黄酮的工艺优选

更新时间：2024-01-04

- HP-20型大孔树脂富集纯化南山茶花总黄酮的工艺优选
- Optimization of Enrichment and Purification Technology for Total Flavonoids from Flowers of by HP-20 Macroporous Resin
- 中国实验方剂学杂志 2013年19卷第7期页码：11-13
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(30772714)
- DOI：10.11653/zgsyfjxzz2013070011
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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尚志春, 宋明明, 史丽颖, 等. HP-20型大孔树脂富集纯化南山茶花总黄酮的工艺优选[J]. 中国实验方剂学杂志, 2013,19(7):11-13.

SHANG Zhi-chun, SONG Ming-ming, SHI Li-ying, et al. Optimization of Enrichment and Purification Technology for Total Flavonoids from Flowers of by HP-20 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 11-13.
尚志春, 宋明明, 史丽颖, 等. HP-20型大孔树脂富集纯化南山茶花总黄酮的工艺优选[J]. 中国实验方剂学杂志, 2013,19(7):11-13. DOI： 10.11653/zgsyfjxzz2013070011.

SHANG Zhi-chun, SONG Ming-ming, SHI Li-ying, et al. Optimization of Enrichment and Purification Technology for Total Flavonoids from Flowers of by HP-20 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 11-13. DOI： 10.11653/zgsyfjxzz2013070011.

摘要

目的: 优选HP-20型大孔树脂富集纯化南山茶花总黄酮的工艺参数。方法: 以总黄酮含量和洗脱率为指标

采用单因素试验考察HP-20型大孔吸附树脂的静态饱和吸附量、动态饱和吸附量、乙醇体积分数、乙醇用量等工艺参数。结果: HP-20型大孔树脂对南山茶花总黄酮的静态饱和吸附量50.5 mg·g-1。最佳纯化工艺为上样液体积9 BV

分别用3 BV去离子水及15%乙醇洗除杂质

35%乙醇10.5 BV以2 BV·h-1流速洗脱。总黄酮纯度达54.24%

总黄酮洗脱率29.83%。结论: HP-20型大孔吸附树脂对南山茶花总黄酮具有良好的纯化效果

优选的工艺合理、稳定可行。

Abstract

Objective: To optimize purification technology parameters of total flavonoids from flowers of Camellia semiserrata with HP-20 macroporous resin. Method: With elution ratio and purity of total flavonoids as indexes

technology parameters of HP-20 macroporous resin were investigated by single factor test

including static saturated adsorption amount

dynamic saturated adsorption amount

the concentration and amount of alcohol

et al. Result: Static saturated adsorption amount was 50.5 mg per gram resin.Optimum purification technology was as following:the volume of sample liquid 9 BV

washed impurity with 3 BV of deionized water and 3 BV of 15% ethanol

eluted with 10.5 BV of 35% ethanol at flow rate of 2 BV·h-1.Under these process conditions

purity of total flavonoids was up to 54.24%

elution rate of total flavonoids was 29.83%. Conclusion: HP-20 macroporous resin had good purification effects on total flavonoids from flowers of C. semiserrata

this optimized purification process was reasonable

stable and feasible.

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