Objective: To establish a method for determining the content of eugenol and imperatorin in compound Nanxing pain paste formed with different stroma

and compare the loss rate of these two components during preparation process. Method: HPLC was adopted performing on a C18 column(4.6 mm×250 mm

5 μm) with a mobile phase of methanol(A)-1% acetic acid (B)

the gradient elution program was as follows:0-30 min

46%A;30-32 min

46%-61%A;32-34 min

61%A;34-42 min

61%-47%A;42-60 min

47%A;The detection wavelength was set at 280 and 300 nm

the column temperature was at 30 ℃.Quality of these prepared three kinds of paste were comprehensively compared with the loss rate of index components during preparation process as indexes. Result: Eugenol was linear in the range of 0.018 31-3.662 7 μg(r=0.999 9)

imperatorin was linear in the range of 0.013 22-0.528 6 μg(r=0.999 9).The loss rate of eugenol in compound Nanxing pain paste formed with different stroma were all large

however the loss rates of imperatorin were all small.Then the loss rates of eugenol in gel ointment of compound Nanxing pain paste were all smaller than solvent and emulsion transdermal patch of compound Nanxing pain paste. Conclusion: This established determination method was accurate and had satisfactory efficacy

it could determine the content of eugenol and imperatorin in these three kinds of compound Nanxing pain paste

and compound Nanxing pain gel ointment was more superior to be prepared as patch.