WEN Ran, GAO Ying, LU Yang, et al. Optimization of Extraction Technology for Guyu Liniment by Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 39-41.
WEN Ran, GAO Ying, LU Yang, et al. Optimization of Extraction Technology for Guyu Liniment by Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 39-41. DOI: 10.11653/zgsyfjxzz2013070039.
Objective: To optimize extraction process of Guyu liniment. Method: Orthogonal test was employed to optimize extraction procedure
including the concentration of ethanol
the amount of solvent
extraction time and extracting times.The content of hydroxy safflor yellow A(HSYA) was used as index which was determined by HPLC. Result: Optimum extracting process for Guyu liniment was as follows:reflux extracted 2 times with 12 times the amount of 70% ethanol
1.5 h per time. Conclusion: This optimized process was stable and feasible with relatively high content of HSYA.