CHEN Xu, DANG Xiao-fang, CAO Sa-li, et al. Screening of Reference Substances for Content Determination of Total Saponins in [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 66-68.
CHEN Xu, DANG Xiao-fang, CAO Sa-li, et al. Screening of Reference Substances for Content Determination of Total Saponins in [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 66-68. DOI: 10.11653/zgsyfjxzz2013070066.
Objective: To investigate the effects of different control samples on content determination of total saponins from Panax notoginseng. Method: With ginsenosides Rg1
ginsenoside Rb1
notoginsenoside R1 and P. notoginseng saponins as reference substance
taking HClO4-vanilin staining method
the content of total saponins in P. notoginseng was determined by ultraviolet spectrophotometry. Result: There were significant differences in determination of the content of total content from P. notoginseng among groups of different references. Conclusion: With UV spectrophotometry as content determination method for total saponins from P. notoginseng
we could choose P. notoginseng saponins as a reference substance
which could provide more reliable experimental data for determining the content of P. notoginseng and its preparations.