SHEN Ling-ling, JI Bin, DUAN Hong-fu, et al. HPLC Fingerprint of Ginger[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 69-73.
SHEN Ling-ling, JI Bin, DUAN Hong-fu, et al. HPLC Fingerprint of Ginger[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 69-73. DOI: 10.11653/zgsyfjxzz2013070069.
Objective: To establish HPLC fingerprint of Ginger
dried Ginger and roasted Ginger to provide evidence for its quality control. Method: A gradient mobile system was applied to the measurement of ten batches of Ginger
dried Ginger and roasted Ginge. Mobile Phase was acetonitrile-water. The UV detection wave length was at 240 nm
and the flow rate was 0.8 mL·min-1. The chromatogram fingerprints were evaluated by the similarity evaluation software published by the committee of codex and calculate the similarity of ten batches of Ginger
dried Ginger and roasted Ginge. Result: The HPLC fingerprint of Ginger was established and six co-possessing peaks were selected separately as the fingerprint peaks of Ginger
dried Ginger and roasted Ginger. The similarity of ten batches of Ginger
dried Ginger and roasted Ginge was respectively more than 0.902
0.911 and 0.929. Conclusion: The instrument precision
stability test and replicate test were in accord with the technical requirements of fingerprint. This method shows the high precision and reliability