Objective: To determine the five alkaloids of different parts in S. flavescens of different growth years. Method: The five alkaloids in S. flavescens were analyzed by HPLC. Result: Five alkaloids of different parts in S. flavescens of different growth years had their distribution law. The content of sophocarpine's distribution in different parts was leaf>stem>lateral root>taproot>reed head; the distribution of matrine was leaf>stem>reed head>lateral root>taproot; the distribution of oxysophocarpine was lateral root>taproot>reed head>stem>leaf; the distribution of sophoridine was lateral root>taproot>leaf>stem>reed head; the distribution of oxymatrine was lateral root>taproot>reed head>stem>leaf. With the increase of growth years

the five alkaloids increased; the fourth year was tend to be gentle. For example

the content of the taproot was: 13.58 mg·g-1 (first year)

20.49 mg·g-1 (second year)

27.74 mg·g-1(third year)

31.32 mg·g-1(forth year). Conclusion: This research can provide data for rational utilization and growth years determination of S. flavescens.