Objective: A near-infrared quantitative method was developed for rapid determination of chlorogenic acid

luteoloside and isochlorogenic acid A in Chrysanthemum morifolium. Method: Near-infrared diffuse reflectance spectroscopy technology was adopted to collect NIR spectra of C. morifolium with HPLC analysis values as reference

the rapid determination method of chlorogenic acid

luteoloside and isochlorogenic acid A was established with partial least squares (PLS). Result: The correlation coefficients

the root-mean-square error of calibration (RMSEC) and the root-mean-square error of validation (RMSEP) of the calibration model for chlorogenic acid

luteoloside and isochlorogenic acid A in C. morifolium were 0.954 93

0.015 7

0.012 7

0.984 50

0.013 1

0.017 6 and 0.998 28

0.009 17

0.005 11

respectively. The correlation coefficient of the true value and prediction value from external validation of 3 ingredients was 0.940 4

0.941 2 and 0.944 6. Conclusion: The method is accurate

fast and simple

which can be applied for the rapid determination of large quantities of number of samples.