HPLC测定山西安泽青翘、老翘主要成分含量

岳晓华; 史静超; 薛慧清; 王一婕; 高丽; 郭羽

doi:10.11653/zgsyfjxzz2013070095

您当前的位置：

首页 >

文章列表页 >

HPLC测定山西安泽青翘、老翘主要成分含量

更新时间：2024-01-04

- HPLC测定山西安泽青翘、老翘主要成分含量
- Quantitative Determination of Major Constituents in Green Fructus Forsythiae and Grown Fructus Forsythiae from Shanxi Anze by HPLC
- 中国实验方剂学杂志 2013年19卷第7期页码：95-97
- 作者机构：
- 作者简介：
- 基金信息：
  
  山西省科技攻关项目(20080311052-1)
- DOI：10.11653/zgsyfjxzz2013070095
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
移动端阅览
岳晓华, 史静超, 薛慧清, 等. HPLC测定山西安泽青翘、老翘主要成分含量[J]. 中国实验方剂学杂志, 2013,19(7):95-97.

YUE Xiao-hua, SHI Jing-chao, XUE Hui-qing, et al. Quantitative Determination of Major Constituents in Green Fructus Forsythiae and Grown Fructus Forsythiae from Shanxi Anze by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 95-97.
岳晓华, 史静超, 薛慧清, 等. HPLC测定山西安泽青翘、老翘主要成分含量[J]. 中国实验方剂学杂志, 2013,19(7):95-97. DOI： 10.11653/zgsyfjxzz2013070095.

YUE Xiao-hua, SHI Jing-chao, XUE Hui-qing, et al. Quantitative Determination of Major Constituents in Green Fructus Forsythiae and Grown Fructus Forsythiae from Shanxi Anze by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 95-97. DOI： 10.11653/zgsyfjxzz2013070095.

摘要

目的: 比较山西安泽青翘与老翘中连翘酯苷、连翘苷

芦丁的含量

为临床上连翘的合理应用、保证临床疗效提供理论依据。方法: 青翘、老翘药材粉末中加甲醇超声波提取45 min;采用HPLC测定3种主要成分的含量

色谱柱为HITACHI C18 (4.6 mm×250 mm

5 μm)

流动相甲醇-0.1%醋酸水

梯度洗脱

流速1 mL·min-1

柱温25 ℃

测定波长280 nm

进样量10 μL。结果: 青翘中连翘酯苷

连翘苷、芦丁的含量分别为2.38%

0.37%

0.26%;老翘中3种成分的含量分别为0.74%

0.20%

0.09%。结论: 山西安泽青翘中连翘酯苷、连翘苷、芦丁的含量均明显高于老翘。

Abstract

Objective: To compare the forsythoside

forsythin and rutin of Green Fructus Forsythiae and Grown Fructus Forsythiae from Shanxi Anze

the main ingredient content can provide the basis for application of forsythia in clinical. Method: The samples were extracted by ultrasonic extraction with methanol

and separated at 25 ℃ on a HITACHI C18 (4.6 mm×250 mm

5 μm) column

eluted with methanol-acetic acid water as mobile phase. Flow rate was set at 1 mL·min-1 and the detection wavelength was set at 280 nm. Sample size was 10 μL. Result: The forsythoside

forsythin and rutin of Green Fructus Forsythiae were 2.38%

0.37%

0.26%; the content of the three components of Grown Fructus Forsythiae was 0.74%

0.20%

0.09%. Conclusion: The main ingredient content of the Green Fructus Forsythiae is higher than the Grown Fructus Forsythiae by HPLC.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

HPLC同时测定连翘花及叶中绿原酸等活性成分的含量

HPLC-DAD同时测定鸡肉参中芦丁、槲皮苷、槲皮素的含量

山西安泽青翘与老翘的氨基酸组分和微量元素分析

UPLC-MS-MS同时测定双黄连粉针中7种活性成分

HPLC-DAD同时测定鸡肉参中芦丁、槲皮苷、槲皮素的含量