Objective: To study the effects of Shenfu injection on the Ca2+-Mg2+-ATP ase

Ca2+-ATP ase

Na+-K+-ATP and Na+

Mg2+

K+

Ca2+ in the myocardial cell injured by nembutal. Method: The myocardial cell is gotten by trypsin digestion and purified by different velocity of adherence. After injured by 0.8% nembutal

the myocardial cell is administrated with 3 different concentrations of Shenfu Injection. Then mensurate the vitality of the Ca2+-Mg2+-ATP ase

Na+-K+-ATP ase and Ca2+-ATP ase and Na+

Mg2+

K+

Ca2+ in the cell 1 h later. Result: The Shenfu injection can increase the strength of the myocardial cell beating injured by nembutal

and enhance the activity of Ca2+-ATP ase and the Ca2+-Mg2+-ATP ase

inhibit the the activity of Na+-K+-ATP

enzyme increase the content of Na+

Mg2+ and decrease the content of K+

Ca2+ in the myocardial cell. Conclusion: The Shenfu injection can protect the myocardial cell injured by nembutal

and the function is relevent to the effects of Shenfu injection on the Ca2+-Mg2+-ATP ase

Na+-K+-ATP ase

Ca2+-ATP ase and the content of Na+

Mg2+

K+

Ca2+ of myocardial cell.