Objective: To observe the anti-inflammatory and analgesic effects of dichloromethane

acetate

and n-butanol extracts from vinegar-processed Cynanchum komarovii and to ensure the active fractions. Method: The mice were divided into 11 groups respectively. The effects were investigated 3 days after drug administration.Hot plate and glacial acetic acid writhing test were adopted in Kunming mice to observe the analgesic effect and the experiment of mice ear swelling induced by xylene and paw edema induced by carrageenan were applied to study on the anti-inflammatory effect. Result: Compared with the model group

all the three extracts of dichloromethane

acetate

and n-butanol (400

800

1 600 mg·kg-1) from vinegar-processed C. komarovii could significantly prolong the time period of paw-licking and reduced the writhing times induced by glacial acetic acid in a dose-dependence (P<0.05

P<0.01). And then the different extracts could inhibit the ear swelling and paw edema

and decrease the content of prostaglandin E2(PGE2) in inflammation tissue. Conclusion: Dichloromethane extract was ensured to be the active fraction. After toxicity attenuation by vinegar-processing

the C. komarovii still has a stronger bioactivity that can prolong the time period of paw-licking and reduced the writhing times induced by glacial acetic acid and inhibit the ear swelling and paw edema

and decrease the content of PGE2 in inflammation tissue. And the anti-inflammatory effect showed a relationship with content of PGE2.