天山雪莲黄酮类成分大孔树脂纯化工艺优选

葛亮; 豆浩然; 张晋; 祁娟娟; 倪健

doi:10.13422/j.cnki.syfix.2014070001

您当前的位置：

首页 >

文章列表页 >

天山雪莲黄酮类成分大孔树脂纯化工艺优选

更新时间：2024-01-04

- 天山雪莲黄酮类成分大孔树脂纯化工艺优选
- Optimization of Purification Technology for Total Flavonoids from Saussureae Involucratae Herba by Macroporous Resin
- 中国实验方剂学杂志 2014年20卷第7期页码：1-3
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81173563）;国家“重大新药创制”科技重大专项（2012ZX09103201-026）;北京中医药大学复方中药制药创新团队基金项目（2011-CXTD-13）;新疆维吾尔自治区重点实验室科研开放课题（XJDX0910-2012-4）
- DOI：10.13422/j.cnki.syfix.2014070001
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
移动端阅览
葛亮, 豆浩然, 张晋, 等. 天山雪莲黄酮类成分大孔树脂纯化工艺优选[J]. 中国实验方剂学杂志, 2014,20(7):1-3.

GE Liang, DOU Hao-ran, ZHANG Jin, et al. Optimization of Purification Technology for Total Flavonoids from Saussureae Involucratae Herba by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 1-3.
葛亮, 豆浩然, 张晋, 等. 天山雪莲黄酮类成分大孔树脂纯化工艺优选[J]. 中国实验方剂学杂志, 2014,20(7):1-3. DOI： 10.13422/j.cnki.syfix.2014070001.

GE Liang, DOU Hao-ran, ZHANG Jin, et al. Optimization of Purification Technology for Total Flavonoids from Saussureae Involucratae Herba by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 1-3. DOI： 10.13422/j.cnki.syfix.2014070001.

摘要

目的：优选天山雪莲总黄酮的大孔树脂纯化工艺。方法：以芦丁为指标成分，通过静态吸附-洗脱试验筛选大孔树脂型号，采用单因素试验考察上样量、上样流速、洗脱流速、洗脱剂用量对天山雪莲黄酮纯化工艺的影响。结果：X-5型大孔树脂纯化效果最好，最佳纯化工艺条件为上样液质量浓度50 g·L-1，上样量6 BV，上样流速3 BV·h-1，加水3 BV洗去水溶性杂质，加20%乙醇6 BV除去其他杂质，加40%乙醇5 BV洗脱，洗脱流速3 BV·h-1，收集40%乙醇洗脱液；树脂纯化前后提取物中芦丁质量分数由1.07%提高至12.14%。结论：X-5型树脂可用于富集天山雪莲中黄酮类成分。

Abstract

Objective: To optimize purification technology of total flavonoids from Saussureae Involucratae Herba by macroporous resin. Method: Taking rutin as index ingredient

macroporous resin model was screened by static adsorption-elution test

single factor tests were adopted to investigate effects of sample amount

sample flow rate

elution rate and other factors on purification technology of total flavonoids from Saussureae Involucratae Herba. Result: X-5 macroporous resin showed the best separation efficiency by compared with other resins

its optimum purification technology were as follows:the concentration of sample solution 50 g·L-1

sample volume of 6 BV

adsorption rate of 3 BV·h-1

washed impurities with 3 BV of water

removed other purities with 6 BV of 20% ethanol

eluted with 5 BV of 40% ethanol

elution velocity of 3 BV·h-1

collected 40% ethanol eluent; After purified

the content of rutin increased from 1.07% in extract to 12.14% in ethanol eluent. Conclusion: X-5 macroporous resin could purify total flavonoids from Saussureae Involucratae Herba.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

栀子总环烯醚萜和总西红花苷的提取纯化工艺考察

小檗皮总生物碱提取物的大孔树脂纯化工艺与质量标准考察

大孔树脂分离纯化楮叶总黄酮的工艺优选

Plackett-Burman联用正交设计优选长叶胡颓子总三萜酸纯化工艺

棉酚的大孔吸附树脂纯化工艺优选