响应面法优化雷公藤中雷公藤甲素的超声提取工艺

朱锡龙; 王兵; 杨光毅; 魏梨霞; 纪晓玲; 蔡慧真; 褚克丹

doi:10.13422/j.cnki.syfix.2014070008

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响应面法优化雷公藤中雷公藤甲素的超声提取工艺

更新时间：2024-01-04

- 响应面法优化雷公藤中雷公藤甲素的超声提取工艺
- Optimization of Ultrasonic Extraction Process for Triptolide from by Response Surface Methodology
- 中国实验方剂学杂志 2014年20卷第7期页码：8-12
- 作者机构：
- 作者简介：
- 基金信息：
  
  “十二五”国家科技支撑计划项目（2011BAI01B05）;福建省科技厅重大专项（2009YZ0001-1-1）;国家级大学生创新创业训练计划项目（201310393018）
- DOI：10.13422/j.cnki.syfix.2014070008
  中图分类号：
- 纸质出版日期：2014
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朱锡龙, 王兵, 杨光毅, 等. 响应面法优化雷公藤中雷公藤甲素的超声提取工艺[J]. 中国实验方剂学杂志, 2014,20(7):8-12.

ZHU Xi-long, WANG Bing, YANG Guang-yi, et al. Optimization of Ultrasonic Extraction Process for Triptolide from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 8-12.
朱锡龙, 王兵, 杨光毅, 等. 响应面法优化雷公藤中雷公藤甲素的超声提取工艺[J]. 中国实验方剂学杂志, 2014,20(7):8-12. DOI： 10.13422/j.cnki.syfix.2014070008.

ZHU Xi-long, WANG Bing, YANG Guang-yi, et al. Optimization of Ultrasonic Extraction Process for Triptolide from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 8-12. DOI： 10.13422/j.cnki.syfix.2014070008.

摘要

目的：优选雷公藤中雷公藤甲素的超声提取工艺。方法：以雷公藤甲素得率为指标，采用响应面设计考察料液比、乙醇体积分数、超声时间及超声温度对雷公藤甲素提取工艺的影响。采用HPLC测定雷公藤甲素含量，流动相乙腈-水（33：67），检测波长218 nm。结果：最佳提取工艺为料液比1：12，乙醇体积分数70%，提取温度35 ℃，提取时间30 min；雷公藤甲素得率达0.037 9 mg·g-1，与模型预测值（0.038 6 mg·g-1）较为接近。结论：优选的超声提取工艺稳定可靠，与常规回流提取法相比优势明显。

Abstract

Objective: To optimize ultrasonic extraction process of triptolide from Tripterygium wilfordii. Method: With yield of triptolide as index

response surface methodology was adopted to optimize extraction technology by taking material-liquid ratio

ethanol concentration

extraction time and ultrasonic temperature as factors.HPLC was adopted to determine the content of triptolide with mobile phase of acetonitrile-water(33:67) and detection wavelength at 218 nm. Result: Optimal extraction conditions were as follows:solid-liquid ratio of 1:12

ethanol concentration 70%

extraction temperature at 35 ℃

extraction time 30 min;under these conditions

yield of triptolide was 0.037 9 mg·g-1

which was close to the predicted value(0.038 6 mg·g-1). Conclusion: This optimized extraction process was stable and reliable

which had obvious advantages by comparing with conventional reflux extraction method.

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