锦灯笼果实提取工艺优选及抗氧化活性考察

宗颖; 王志颖; 孙佳明; 杜锐

doi:10.13422/j.cnki.syfix.2014070015

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锦灯笼果实提取工艺优选及抗氧化活性考察

更新时间：2024-01-04

- 锦灯笼果实提取工艺优选及抗氧化活性考察
- Optimization of Extraction Technology for Physalis Calyx Seu Fructus Fruit and Investigation of its Antioxidant Activity
- 中国实验方剂学杂志 2014年20卷第7期页码：15-18
- 作者机构：
- 作者简介：
- 基金信息：
  
  吉林省教育厅科学技术研究项目（吉教科合字[2012]第50号）
- DOI：10.13422/j.cnki.syfix.2014070015
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- 纸质出版日期：2014
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宗颖, 王志颖, 孙佳明, 等. 锦灯笼果实提取工艺优选及抗氧化活性考察[J]. 中国实验方剂学杂志, 2014,20(7):15-18.

ZONG Ying, WANG Zhi-ying, SUN Jia-ming, et al. Optimization of Extraction Technology for Physalis Calyx Seu Fructus Fruit and Investigation of its Antioxidant Activity[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 15-18.
宗颖, 王志颖, 孙佳明, 等. 锦灯笼果实提取工艺优选及抗氧化活性考察[J]. 中国实验方剂学杂志, 2014,20(7):15-18. DOI： 10.13422/j.cnki.syfix.2014070015.

ZONG Ying, WANG Zhi-ying, SUN Jia-ming, et al. Optimization of Extraction Technology for Physalis Calyx Seu Fructus Fruit and Investigation of its Antioxidant Activity[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 15-18. DOI： 10.13422/j.cnki.syfix.2014070015.

摘要

目的：优选锦灯笼果实的提取工艺并考察提取物的体外抗氧化活性。方法：以总黄酮、总酚酸提取量为指标，通过单因素试验和正交试验考察乙醇体积分数、提取时间及料液比对锦灯笼果实醇提工艺的影响，考察提取物对ABTS自由基的清除能力。结果：最佳提取工艺为加20倍量85%乙醇提取2次，每次0.5 h；总黄酮和总酚酸平均提取量分别为0.618，1.152 mg·g-1，RSD依次为1.66%，1.19%。结论：结合药效学试验筛选的提取工艺更加科学合理，锦灯笼果实具有一定的体外抗氧化能力。

Abstract

Objective: To optimize extraction process of Physalis Calyx Seu Fructus fruit and investigate antioxidant activity of its extract. Method: With extraction amounts of total flavonoids and total phenolic acids as index

orthogonal test and single factor tests were employed to optimize ethanol extraction process of Physalis Calyx Seu Fructus fruit by taking ethanol concentration

extraction time and solid-liquid ratio as factors

and antioxidant activity of extract was investigated by observing its ability of scavenging ABTS free radical. Result: Optimum extraction technology was as follows:extracted twice with 20 times the amount of 85% ethanol

0.5 hour per time;Average extraction amounts of total flavonoids and total phenolic acids were 0.618

1.152 mg·g-1 with RSD of 1.66% and 1.19%

respectively. Conclusion: It was reasonable to optimize extraction technology by taking pharmacodynamics test as index

Physalis Calyx Seu Fructus fruit had a certain in vitro antioxidant activity.

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