Objective: To optimize ultrafine grinding technology for cell wall disruption of Dendrobii Officinalis Caulis. Method: With particle size

cell wall-broken rate and the content of total polysaccharides as comprehensive evaluation index

L16(43×26) orthogonal test was adopted to optimize ultrafine grinding process by taking grinding time

amount and particle size into mill

grinding temperature and moisture content of materials as factors. Result: Optimum grinding process was as follows:particle size into mill 80 mesh

moisture content of materials 4%-6%

materials amount into mill 250 g

grinding temperature 0-10 ℃

grinding time 25 min;Average powder yield was 96.1%(RSD 0.24%)

average yield of particle size<75 μm was 96.8%(RSD 0.28%)

average cell wall-broken rate was 98.3%(RSD 0.78%)

average mass fraction of total polysaccharides was 42.2%(RSD 2.85%). Conclusion: This optimized process was reasonable and stable

which provided experimental data for clinical effective application of Dendrobii Officinalis Caulis.