Objective: To develop an analysis method of simultaneously determining [luteolin-7-O-gentibioside (LGT)

luteolin-7-O-β-D-glucopyranoside (LGCOP)

luteolin-7-O-β-D-glucuronopyranoside (LGCRP)

apigenin-7-O-β-D-glucopyranoside (AGCOP)

apigenin-7-O-β-D-glucuronopyranoside (AGCRP)

luteolin (LI) and apigenin (AGI)] in Kudiezi injection by RP-HPLC-DAD. Method: 0.05% formic acid-acetonitrile and 0.05% formic acid-water as the mobile phase

chromatographic column was Waters Symmetry C18(3.9 mm×150 mm

4.6 μm) in gradient elution mode with velocity:1 mL·min-1

column temperature 35 ℃

detection wavelength of 340 nm. Result: There were perfect chromatogram with good separation. All the 7 calibration curves showed a good linear relationship. The recoveries were 99.0%-101.5%

according to the requirement. The developed method was successfully applied for the simultaneous determination of the 7 components (LGT

LGCOP

LGCRP

AGCOP

AGCRP

LI

AGI). After the determination of 8 batch of Kudiezi injection

the content of 7 flavonoids was in 0.157 9-103.4 mg·L-1. Conclusion: The method is convenient

specific

and can be used for the flavonoids constitutes quality control of Kudiezi injection.