Objective: To study the HPLC fingerprint and quantitative analysis of 4 component from extract of Rosa davurica from different producing areas. Method: With an Agilent ZORBAX SB-C18column(4.6 mm×250 mm

5 μm)

gradient elution was performed by mobile phase of 0.5% formic acid-acetonitrile-methanol-tetrahydrofuran; detection wavelength was sat at 370 nm. The flow rate was 1.0 mL·min-1; the column temperature was kept at 30 ℃. The data calculation was performed with similarity evaluation system for chromatographic fingerprint of TCM (Version 2004A). Result: 10 common peaks were found from 18 batches of R. davurica extract HPLC fingerprint

of which 4 peaks were confirmed. Fingerprint provides a scientific similarity of each batch of samples higher than 0.9. Conclusion: This method provides basis for controlling the quality of extract of R. davurica.