Mahemuti·, Maimaitiming, Kelimu·, et al. Study on Antioxidative Activity of Polysaccharide from Uighur Medicine Turnip Seed [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 118-121.
Mahemuti·, Maimaitiming, Kelimu·, et al. Study on Antioxidative Activity of Polysaccharide from Uighur Medicine Turnip Seed [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 118-121. DOI: 10.13422/j.cnki.syfix.2014070118.
Objective: An preliminary study on antioxidant activity of Turnip seed polysaccharide was carried out through radical scavenging experiment in vitro. Method: Several methods include degreasation
water extraction
deproteinization
decolorization and alcohol precipitation were adopted to extract polysaccharide. Four common of antioxidant activity in vitro testing methods were selected
namely scavenging DPPH radical
hydroxyl radical
superoxide anion radical and total reducing ability
to measure the antioxidant capacity of Turnip seed polysaccharide
and ascorbic acid Vit C(Vc) was selected as the positive control. Result: Turnip seed polysaccharide have a certain amount of antioxidant activity
and the extraction rate of is 2.34%. The antioxidant capacity of polysaccharide and its concentration have good dose-effect relationship. Besides
higher concentration can enhance the antioxidant activity. Meanwhile
Turnip seed polysaccharide has strong total reduction ability
but relatively weak superoxide anion radical scavenging capacity. Conclusion: The experiments show that Turnip seed polysaccharide has natural antioxidant capacity
and using turnip seed as raw materials to develop multi-functional carbohydrate foods will have a more realistic practical significance.