Objective: To identify an unknown broken plant sample. Method: In this work

the method included macroscopical identification

microscopic identification

DNA barcoding identification was used. Using the DNA barcoding identification technology

DNA extraction

PCR amplification and sequencing

ITS sequence data were analyzed by three methods about the Blast comparison method

specific point method

system development method. Result: The unknown sample is the seeds of family umbrelliferae plant Foeniculum vulgare Mill. Conclusion: Traditional Chinese medicine authentication technology can solve the species identification problem of unknown sample in the actual work. DNA barcoding technology provide a new method for TCM authentication and play an important role of the identification of unknown sample.