Objective: To study the antioxidant activity of different extracts from Eurotium cristatum in vitro. Method: The petroleum ether

ethyl acetate and n-butanol extracts from E. cristatum were attained by the extraction method of the methanol extract of the E.cristatum. The antioxidant activity of E.cristatum was evaluated by 1

1-diphenyl-2-picrylhydrazyl(DPPH) radical and (ABTS) radical scavenging assay with vitamin C(VC) as the positive control. Result: The petroleum ether extract from E. cristatum showed significant DPPH and ABTS radical scavenging activity

with 50% inhibition concentration(IC50) values of 0.078

0.083 g·L-1

respectively

the positive control VC showed IC50 values of 0.032

0.024 g·L-1

respectively.The ethyl acetate extract exhibited modest radical scavenging activity against DPPH and ABTS radicals

with IC50 values of 0.21

0.13 g·L-1

whereas the n-butanol extract did not display noticeable in vitro activity against above-mentioned radicals. Conclusion: The petroleum ether and ethyl acetate extracts from E. cristatum showed significant antioxidant activity.