Objective: To investigate the effects of Jianpi Huoxue decoction on cyclooxygenase-2(COX-2) and matrix metalloproteinase-2 (MMP-2) expression in rats with precancerous lesions of gastric cancer(PLGC). Method: Sixty rats were randomly divided into the normal group(10) and the modeling group(50).Except normal group

PLGC model was established in other rats for 12 weeks with following. Method: 150 mg·L-1 N-methyl-N-nitro N-nitrosoguanidine(MNNG) solusion was drunk freely

170 mg·L-1 MNNG which contained 0.03% ranitidine at dose of 10 mL·kg-1 was administered once a day

40% alcohol (10 mL·kg-1)was administered twice a week

and irregular diet was given from the 5th week.Forty rats after modeling were randomly divided into the model control group(0.9%NS)

the Weifuchun group(0.86 g·kg-1)

the high-dose

middle-dose and low-dose of Jianpi Huoxue decoction groups(32

16

8 g·kg-1)

8 rats in each group.Each group was given corresponding medicines once a day for 10 weeks.All of the rats were sacrificed by the 22th weekend.The expression of COX-2 and MMP-2 was detected by rapid immunohistochemistry. Result: Compared with the normal group

the COX-2 and MMP-2 expressions were significantly increased in the model group(P<0.01)

respectively. Compared with the model group

the COX-2 and MMP-2 expressions were significantly decreased in the high-dose

middle-dose

low-dose Jianpi Huoxue decoction groups and Weifuchun group(P<0.01 or P<0.05)

respectively.Compared with the Weifuchun group

the COX-2 and MMP-2 expressions were significantly decreased in the high-dose of Jianpi Huoxue decoction group(P<0.05)

respectively.But there was no significant difference in the the COX-2 and MMP-2 expression between the Weifuchun group and the middle-dose and low-dose Jianpi Huoxue decoction groups. Conclusion: The therapeutic effect of Jianpi Huoxue decoction is associated with the reduction of COX-2 and MMP-2 expression in gastric mucosa.