JIE Jing, ZHAO Yue, WANG Hao. Optimization of Ultrasound Extraction Technology for Total Saponins from by Box- Behnken Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 8-11.
JIE Jing, ZHAO Yue, WANG Hao. Optimization of Ultrasound Extraction Technology for Total Saponins from by Box- Behnken Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 8-11. DOI: 10.13422/j.cnki.syfix.2014080008.
Objective: To optimize ultrasound extraction technology of total saponins from Asparagus officinalis. Method: With the content of total saponins as index
effects of ethanol concentration
solid-liquid ratio
ultrasonic time and temperature on extraction technology were investigated by single factor tests and Box-Behnken response surface methodology. Result: Optimum ultrasound extraction technology was as following:ultrasonic extracted 54 min with 15 times the amount of 74% ethanol at 50 ℃;Under these conditions
the average mass fraction of total saponins was 13.059%
which was wery close to the predicted value of 13.185%. Conclusion: This optimized technology was stable and feasible to provide a basis for development and application of total saponins in A. officinalis.