星点设计-响应面法优选枳实总黄酮的大孔吸附树脂纯化工艺

马丽; 陈家仪; 陈稚; 梁巧文; 曹骋; 曾元儿

doi:10.13422/j.cnki.syfix.2014080033

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星点设计-响应面法优选枳实总黄酮的大孔吸附树脂纯化工艺

更新时间：2024-01-04

- 星点设计-响应面法优选枳实总黄酮的大孔吸附树脂纯化工艺
- Optimization of Purification Process for Total Flavonoids in Aurantii Fructus Immaturus with Macroporous Resin by Central Composite Design-Response Surface Methodology
- 中国实验方剂学杂志 2014年20卷第8期页码：33-36
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省大学生创新实验项目（1057211030）
- DOI：10.13422/j.cnki.syfix.2014080033
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
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马丽, 陈家仪, 陈稚, 等. 星点设计-响应面法优选枳实总黄酮的大孔吸附树脂纯化工艺[J]. 中国实验方剂学杂志, 2014,20(8):33-36.

MA Li, CHEN Jia-yi, CHEN Zhi, et al. Optimization of Purification Process for Total Flavonoids in Aurantii Fructus Immaturus with Macroporous Resin by Central Composite Design-Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 33-36.
马丽, 陈家仪, 陈稚, 等. 星点设计-响应面法优选枳实总黄酮的大孔吸附树脂纯化工艺[J]. 中国实验方剂学杂志, 2014,20(8):33-36. DOI： 10.13422/j.cnki.syfix.2014080033.

MA Li, CHEN Jia-yi, CHEN Zhi, et al. Optimization of Purification Process for Total Flavonoids in Aurantii Fructus Immaturus with Macroporous Resin by Central Composite Design-Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 33-36. DOI： 10.13422/j.cnki.syfix.2014080033.

摘要

目的：优选枳实总黄酮的大孔树脂纯化工艺。方法：选择吸附时间、乙醇体积分数、洗脱用量为自变量，总黄酮吸附-洗脱率为因变量，在单因素试验基础上，采用星点设计-响应面法优选大孔树脂纯化工艺。结果：选择AB-8型大孔树脂，最佳纯化工艺为上样液质量浓度3.5 g·L-1，pH 6.0，上样量0.6 BV，上样速率1 BV·h-1，水洗用量1 BV，洗脱速率2 BV·h-1，吸附时间77 min，乙醇体积分数88%，洗脱剂用量2.4 BV；枳实总黄酮吸附-洗脱率达93.0%，与预测值（92.4%）偏差较小。结论：采用星点设计-响应面法优选的大孔树脂纯化工艺条件稳定可行，预测性好。

Abstract

Objective: To optimize purification process of total flavonoids in Aurantii Fructus Immaturus by macroporous resin. Method: Independent variables were adsorption time

ethanol concentration and eluent volume

while dependent variables was adsorption-elution rate of total flavonoids

central composite design-response surface methodology was adopted to optimize purification technology on the basis of single factor tests. Result: AB-8 macroporous resin was selected

optimum purification process were as follows:added 0.6 BV of sample solution with concentration of 3.5 g·L-1 and pH 6.0

sample rate 1 BV·h-1

adsorption time 77 min

water consumption 1 BV

elution rate of 2 BV·h-1

eluted with 2.4 BV of 88% ethanol.Adsorption-elution rate of total flavonoids was up to 93.0%

which had a small deviation with the predicted value of 92.4%. Conclusion: Optimized purification technology was stable and feasible with high predictability.

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