Objective: To study the effect of ampelopsin on the HSC-T6 cell proliferation rate and the content of collage and cytokine Method: Effect of ampelopsin on the cell (HSC-T6) proliferation rate. High glucose DMEM medium containing 10% fetal bovine serum was used to prepare HSC-T6 cells 3×104 cells/mL suspension 100 μL/well

which was seeded in 96-well culture plates. The control group

colchicine 1.0 mg·L-1 group and 100.0

75.0

50.0

25.0

12.5 mg·L-1

5 different concentrations of ampelopsin groups were designed. Cells were cultured at 37 ℃ 5%CO2 for 48 h. The cell proliferation inhibition rate was measured by MTT。Effect of ampelopsin on the content of collage(Ⅰ

Ⅲ

Ⅳ) and cytokine (TGF-β1

PDGF) was dected by ELISA. Result: Ampelopsin at 100.0

75.0 mg·L-1 might effectively inhibit proliferation of HSC-T6 cells

and the non-toxic drug concentration was 50.0 mg·L-1

the contents of collage Ⅰ

Ⅲ

Ⅳ and TGF-β1

PDGF in HSC-T6 cells were significantly lower than control group at 50.0 mg·L-1 of ampelopsin (P<0.05)

the contents of collage Ⅲ in HSC-T6 cells was significantly lower than control group by ampelopsin at 25.0 mg·L-1(P<0.05). Conclusion: Ampelopsin could inhibit the proliferation of HSC-T6 and reduce the content of collage Ⅰ

Ⅲ

Ⅳ and TGF-β1

PDGF.