Objective: To compare the formulations of Danggui Buxue decoction

granule

oral liquid of 3 different preparation of various composition differences

which is used for the contradistinction of affects for chemical compositions of different pharmaceutical technologies. Method: 9 samples of formulations of Danggui Buxue were extracted by 5% methanol-water solution. Sample solutions were determined by Lab Alliance high performance liquid chromatograph instrument equipped with Kromasil C18 column and UV detector

and gradient eluted with methanol-0.4% formic acid aqueous solution as mobile phase. The flow rate was set to 1 mL·min-1

and the wavelength for detection was set to 280 nm. Through the comparison of HPLC characteristic spectrum

and the use of traditional Chinese medicine fingerprint digital information processing technology of Danggui Buxue Decoction

difference analysis on granules

specific

main components and unknown components was performed. Result: It had obvious characteristics for the common peaks of the HPLC chromatographic profiles for the formulations of Danggui Buxue. 15 common peaks were detected. The similarity indexes had some differences between different formulations of Danggui Buxue preparations. The amount and areas of peaks in dispensing granules and oral liquor had different degrees of reduction

the proportion of various components with different. Conclusion: Compared with the decoction

chemical compositions in Danggui Buxue dispensing granules and oral liquor are subject to varying degrees of loss in preparation process

and may affect the clinical efficacy. The HPLC chromatographic profiles method appeared to be simple

accurate and can be used for the quality researches of the formulations of Danggui Buxue.