不同产地穿心莲的含量测定、化学指纹图谱及抑菌活性评价

王艳辉; 王伽伯; 郝庆秀; 张雅铭; 赵艳玲; 格小光; 郭兰萍; 黄璐琦; 肖小河

doi:10.13422/j.cnki.syfix.2014090077

您当前的位置：

首页 >

文章列表页 >

不同产地穿心莲的含量测定、化学指纹图谱及抑菌活性评价

更新时间：2024-01-04

- 不同产地穿心莲的含量测定、化学指纹图谱及抑菌活性评价
- Evaluation of Chemical Contents, Fingerprint and Antimicrobial Activity of (Burm. f.) Nees Harvested in Different Regions
- 中国实验方剂学杂志 2014年20卷第9期页码：77-82
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技支撑计划项目（2012BAI29B02）;国家公益性行业科研专项（201207002）;中央本级课题（20603020101）
- DOI：10.13422/j.cnki.syfix.2014090077
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
移动端阅览
王艳辉, 王伽伯, 郝庆秀, 等. 不同产地穿心莲的含量测定、化学指纹图谱及抑菌活性评价[J]. 中国实验方剂学杂志, 2014,20(9):77-82.

WANG Yan-hui, WANG Jia-bo, HAO Qing-xiu, et al. Evaluation of Chemical Contents, Fingerprint and Antimicrobial Activity of (Burm. f.) Nees Harvested in Different Regions[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(9): 77-82.
王艳辉, 王伽伯, 郝庆秀, 等. 不同产地穿心莲的含量测定、化学指纹图谱及抑菌活性评价[J]. 中国实验方剂学杂志, 2014,20(9):77-82. DOI： 10.13422/j.cnki.syfix.2014090077.

WANG Yan-hui, WANG Jia-bo, HAO Qing-xiu, et al. Evaluation of Chemical Contents, Fingerprint and Antimicrobial Activity of (Burm. f.) Nees Harvested in Different Regions[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(9): 77-82. DOI： 10.13422/j.cnki.syfix.2014090077.

摘要

目的：从化学和生物两方面综合考察不同产地穿心莲药材品质差异。方法：采用微量量热法比较13批不同产地穿心莲药材的抗痢疾杆菌生物活性。同时建立穿心莲的UPLC指纹图谱，采用偏最小二乘法（PLS）回归分析抑菌活性与化学成分之间的相关性。结果：13批不同产地的穿心莲药材使痢疾杆菌生长代谢的时间延长，生长速率常数减小，表明不同产地穿心莲对痢疾杆菌的生长代谢均有不同程度的抑制作用，且以西双版纳产地的穿心莲药材抑菌效果最好。此外，通过偏最小二乘法回归分析找到了四个与抑菌活性密切相关的化学成分。结论：不同产地穿心莲药材质量差异较大，云南、海南、广西和四川综合质量较好。生物活性测定结合化学指纹图谱评价药材质量的方法，可准确、可靠地评价不同产地穿心莲药材的质量，为其质量控制提供依据。

Abstract

Objective: The aim of the study was to evaluate the quality of Andrographis paniculata (Burm. f.) Nees harvested in different regions of China. Method: The inhibitory effects of A. paniculata on Shigella dysenteriae growth were investigated by microcalorimetry. UPLC fingerprints of the A. paniculata samples were acquired and then analyzed the correlation with the antimicrobial activity by partial least squares (PLS) analysis. Result: Compared with the control group

all A. paniculata samples from different regions showed inhibitory effects to S. dysenteriae with extending of growth time and decrease of metabolism. And the samples from Yunnan Xishuangbanna had stronger antimicrobial effects than others. According to PLS analysis

there were four chemical contents correlative to the activity of antimicrobial effect. Conclusion: The harvest region had significant influence to the quality of A. paniculata and Yunnan

Hainan

Sichuang and Guangxi were the good harvest regions. The combined evaluation of biological activity and chemical fingerprint provides ability to integrally evaluate the quality of A. paniculata.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

UPLC快速分析穿心莲药材及制剂中内酯成分

穿心莲内酯药理作用及机制研究进展

HPLC法测定妇科千金胶囊中穿心莲内酯和脱水穿心莲内酯的含量

高效液相色谱法测定穿心莲内酯掩味树脂复合物的含量

穿心莲滴丸中穿心莲内酯的体外溶出度研究