壮药大头陈中桦木酸的分离鉴定及含量测定

陆国寿; 黄秋洁; 叶勇; 卢文杰; 牙启康; 黄伟城

doi:10.13422/j.cnki.syfix.2014090090

您当前的位置：

首页 >

文章列表页 >

壮药大头陈中桦木酸的分离鉴定及含量测定

更新时间：2024-01-04

- 壮药大头陈中桦木酸的分离鉴定及含量测定
- Separation and Determination of Betulinic Acid in (Lour.) Merr. by HPLC
- 中国实验方剂学杂志 2014年20卷第9期页码：90-92
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西卫生厅中医药科技专项（GZKZ1135）
- DOI：10.13422/j.cnki.syfix.2014090090
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
移动端阅览
陆国寿, 黄秋洁, 叶勇, 等. 壮药大头陈中桦木酸的分离鉴定及含量测定[J]. 中国实验方剂学杂志, 2014,20(9):90-92.

LU Guo-shou, HUANG Qiu-jie, YE Yong, et al. Separation and Determination of Betulinic Acid in (Lour.) Merr. by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(9): 90-92.
陆国寿, 黄秋洁, 叶勇, 等. 壮药大头陈中桦木酸的分离鉴定及含量测定[J]. 中国实验方剂学杂志, 2014,20(9):90-92. DOI： 10.13422/j.cnki.syfix.2014090090.

LU Guo-shou, HUANG Qiu-jie, YE Yong, et al. Separation and Determination of Betulinic Acid in (Lour.) Merr. by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(9): 90-92. DOI： 10.13422/j.cnki.syfix.2014090090.

摘要

目的：对大头陈中的化学成分进行研究；采用HPLC法对大头陈中桦木酸的含量进行测定。方法：采用溶剂提取、萃取、硅胶色谱柱分离纯化，利用色谱技术进行化学结构鉴定；HPLC法进行含量测定的色谱条件为：采用SHISEIDO-SPOLAR C18（4.6 mm×250 mm，5 μm），流动相甲醇-0.2%磷酸水溶液（82：18），流速1.0 mL·min-1，检测波长205 nm，柱温35 ℃。结果：从大头陈药材中分离得到一化合物，经鉴定为桦木酸；桦木酸在0.048~0.48 μg与其峰面积呈良好的线性关系；平均回收率99.84%，RSD 1.35%。结论：该化合物首次从该植物中分离得到。所建立的HPLC简便、准确、重复性好。结果准确可靠，可用于大头陈中桦木酸的测定。

Abstract

Objective: To study the chemical constituents of Adenosma indianum (Lour.) Merr. and the contents of betulinic acid in Adenosma indianum (Lour.) Merr. by HPLC. Method: The chemical constituents were isolated by column chromatography over silica ge1.The Structures of the compound was elucidated by spectroscopic and chromatographic analysis.SHISEIDO-SPOLAR C18 column (4.6 mm×250 mm

5 μm) was used with methanol-0.2% phosphoric acid (82:18) as mobile phase. The flow rate was 1.0 mL·min-1.The UV detection wavelength was 205 nm and the column temperature was 35 ℃. Result: The compound was identified as betulinic acid.The linear response range of betulinic acid were 0.048-0.48 μg

respectively.The average recovery of this compound was 99.84%

RSD 1.35%. Conclusion: The betulinic acid was isolated from Adenosma indianum (Lour.) Merr. for the first time.This established method in this study is simple

reliable

reproducible and accurate for the analysis of betulinic acid in Adenosma indianum (Lour.) Merr.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

响应面法优化连翘中桦木酸超声辅助提取工艺