DANG Xiao-fang, CAO Sa-li, QI Juan-juan, et al. Optimization of Determination for Total Flavonoids in [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(9): 96-98.
DANG Xiao-fang, CAO Sa-li, QI Juan-juan, et al. Optimization of Determination for Total Flavonoids in [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(9): 96-98. DOI: 10.13422/j.cnki.syfix.2014090096.
Objective: To establish a method for determination of the content of total flavonoids in Euonymus alatus. Method: Selecting rutin as a reference substance
single factor tests were adopted to optimize colorimetry methods and conditions
determination of the content of total flavonoids was established and optimized its extraction method. Result: Hydrochloric acid-Mg reaction assay was adopted
rutin had a liner relationship with absorbance in the range of 0.021-0.104 mg (r=0.997)
average recovery was 99.424% with RSD of 2.220%. Conclusion: HCl-Mg colorimetry method appeared to be an accurate
reliable and repeatable system for determination of total flavonoids in E. alatus.Total flavonoids in E.alatus should employ reflux extraction method.