Objective: To observe the therapeutic effect of optimized Huoxue Tongfu (OHT) formula for peritoneal adhesion

to ensure the optimum dosage. Method: Forty-eight male rats were divided randomly into 6 groups:pseudo-operation group

model group

low dose OHT formula group

medium dose OHT formula group and high dose (OHT) formula group and Simo decoction group. All the rats were molded into peritoneal adhesion by file except those in pseudo-operation group. We observed and estimated the peritoneal adhesion and general situation of rats

Elisa method was used to measure the serum level of interleukin (IL)-8

IL-10 and tumor necrosis factor-α (TNF-α)

and reverse transcription-polymerase chain reaction for the expression of collagen Ⅰ (Col-Ⅰ) mRNA in adhesional tissue. Result: Compared to pseudo-operation group

in the model group all layers of the intestinal wall showed vessel dilatation

edema

serous layer obviously lymphocyte infiltration

hyperplasia of fibroblasts. The muscular layer structure were morphologically complete

the placenta percreta were slightly thickened and no obvious hyperplasia were found in the fibrous connective tissue in placenta percreta of all the other groups. Compared with the model group

the medium dosage group of optimized Huoxue Tongfu formula

serum IL-8 and TNF-α levels were (154.2±16.2)

(58.2±6.4) ng·L-1

but the level of IL-10 (58.2±6.4) ng·L-1increased

while decreased expression of Col-ⅠmRNA adhesion tissues (0.41±0.11)

there was significant statistical significance (P<0.05 or P<0.01). Simo decoction group could also reduce abdominal adhesion score and serum IL-8 and TNF-α levels

elevated IL-10 levels

but compared with the medium dosage group of OHT formula the difference marked (P<0.05). Conclusion: The medium dosage group of OHT formula can suppress inflammation and relieve peritoneal adhesion by regulating the serum level of IL-8

IL-10 and TNF-α

reducing the deposition of the Col-ⅠmRNA of extracellular matrix.