Objective: To observe the effect of Huatan Tongluo decoction on aquaporin-4 (AQP-4) protein expression of different brain tissues and S100β of blood serum in rats which use rt-PA thrombolysis after the acute cerebral infarction. Method: SD rats 240 were randomly divided into six groups

sham operation group

model group

rt-PA group

rt-PA Huatan Tongluo of Chinese medicine low dose group (3.6 g·kg-1)

rt-PA Huatan Tongluo of Chinese medicine middle dose group (7.2 g·kg-1)

rt-PA Huatan Tongluo of Chinese medicine high dose group (14.4 g·kg-1). Using the embolus method prepares the MCAO rat model. rt-PA group and rt-PA Huatan Tongluo group use rt-PA thrombolytic therapy after thrombosis. In 6 hours group the Chinese medicine were given one time

after making mode successfully.In 24 hours group

given two times

after making mode successfully and 2 hours were killed.In 72 hours

given six times

from making mode successfully

two times one day

lasting three days.In 7 day group;given fourteen times

from making mode successfully

two times one day

lasting seven days. Then respectively observe the AQP-4 protein expression in rat cortex and hippocampus by Western blot method

and S100β of blood serum by ELISA method at 6

24

72 h

7 days. Result: Every experimental group in different phases

the AQP-4 protein expression in different brain tissue and S100β of blood serum were highest at 6

24 h maximum

72 h

7 d increased gradually to the level of 6 h. Compared with the model group at the same time the AQP-4 protein expression of middle dose group and high dose group of Chinese traditional medicine were significantly decreased at 24 h or 72 h or 7 d (P<0.05). There was no obvious concentration

but the overall trend showed that Chinese medicine middle dose group and high dose group can obviously decrease the expression of AQP-4.The S100β protein expression didn't show any significant differences in any group

but all have reduced in different degree

and the downward trend is more obvious in Chinese traditional medicine groups. Conclusion: The blood brain barrier structure and function was damaged most seriously at 24 h phase after thrombolysis treatment.Huatan Tongluo combined with rt-PA therapy can reduce AQP-4 expression of different brain tissues and S100β content of serum. So that it can protect the hemorrhagic transformation after thrombolytic treatment of acute cerebral infarction.