Objective: To study the effects of Mongolian pharmaceutical Betel Shisanwei ingredients pill (BSIP) on function of nerve-endocrine-immune in chronic unpredictable stress depression rat and therapeutic mechanism of depression. Method: Sixty male Wistar rats were randomly divided into six groups according to the sugar consumption test and body weight (10 rats in each group) :Normal control group

model group

Fluoxetine group (3.3 mg·kg-1) and high dose

medium dose and low dose group (0.25

0.5

1 g·kg-1) of BSIP.Except the normal control

the other groups were treated with the chronic unpredictable mild stress stimulation combined with lonely raising for 28 days.10 mL·kg-1 drugs was given to the rats

once daily

continuously for 28 days. After the experiment blood samples were collected and enzyme-linked immunosorbent (ELISA) was used to detect corticotropin releasing hormone (CRH)

adrenocorticotropic hormone (ACTH)

corticosterone (CORT)

3

5

3'-triiodothyronine (T3)

3

5

3'

5'-tetraiodothyronine (T4)

thyroid stimulating hormone (TSH)

interleukin-2 (IL-2)

the content of interleukin-6 (IL-6)

and take the thymus

spleen to calculate thymus index

and spleen index. Result: compared with normal control group

model group rats serum CRH

ACTH

CORT

T3

IL-6 levels increased significantly (P<0.01)

the TSH levels

spleen index significantly decreased (P<0.01) ;Compared with model group

in the fluoxetine group and BSIP group

the serum CRH

ACTH

CORT

T3

IL-6 content significantly decreased (P<0.01 or P<0.01) ;the TSH levels increased significantly (P<0.05 or P<0.01) ;in the BSIP group

the spleen index increased significan tly (P<0.01)

especially in the BSIP high dose group

it was significant. Conclusion: BSIP can adjust the depression model rats hypothalamus-pituitary-adrenal axis and hypothalamus-pituitary-thyroid axis function

and immune function

this could be one of mechanisms.