LIU Ji-jin~, HUANG Fu-xi, XIONG Ying. Simultaneous Determination of Seven Components in Huoluo Oil by Capillary GC[J]. Chinese journal of experimental traditional medical formulae, 2006, (10): 16-18.
LIU Ji-jin~, HUANG Fu-xi, XIONG Ying. Simultaneous Determination of Seven Components in Huoluo Oil by Capillary GC[J]. Chinese journal of experimental traditional medical formulae, 2006, (10): 16-18. DOI: 10.13422/j.cnki.syfjx.2006.10.009.
Objective:To develop a Capillary GC method to determine the seven components
α-pinene
eucalyptol
camphor
menthol
methyl salicylate
cinnamia aldehyde and eucalyptal in Huoluo oil.Method:A fused silica capillary column was used.The intial temperature of column was kept at 90℃ for 1 min
then raised to 180℃ at the rate of 6℃·min
-1
finally to 250℃ at the rate of 25℃·min
-1
and maintained the temperature for 5 min
the carry gas was nitrogen.The injector and detector temperature was 220℃ and 260℃.Injections were performed in the splitless mode and an FID was used as the detector.Results:The standards curves of α-pinene
eucalyptol
camphor
menthol
methyl salicylate
cinnamia aldehyde
eucalyptal are linear within the range of 6.94~41.64μg·mL
-1
1.00~6.01 μg·mL
-1
6.86~41.1 μg·mL
-1
24.1~144.5 μg·mL
-1
24.09~144.55μg·mL
-1
24.16~144.98μg·mL
-1
1.03~6.17μg·mL
-1
1.04~6.25μg·mL
-1
and RSD was 1.8
1.1
0.9
0.7
1.1
1.7
1.1%.Conclusion:This method is simple and accurate