枸杞、黄芪对大鼠睾丸支持细胞抑制素合成的影响

赵龙坡; 徐铮; 张文; 孙辉臣; 汤菲

doi:10.13422/j.cnki.syfjx.2007.01.023

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枸杞、黄芪对大鼠睾丸支持细胞抑制素合成的影响

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- 枸杞、黄芪对大鼠睾丸支持细胞抑制素合成的影响
- The Effect of Fructus Lycii and Radix Astragali on Synthesis of Inhibin in Sertoli Cells of Rat Testis
- 中国实验方剂学杂志 2007年第1期页码：58-61
- 作者机构：
  
  1. 白求恩国际和平医院
  2. 白求恩国际和平医院河北石家庄050082
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2007.01.023
  中图分类号： R285.5
- 纸质出版日期：2007
- 稿件说明：
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[1]赵龙坡,徐铮,张文,孙辉臣,汤菲.枸杞、黄芪对大鼠睾丸支持细胞抑制素合成的影响[J].中国实验方剂学杂志,2007(01):58-61.

ZHAO Long-po, XU Zheng, ZHANG Wen, et al. The Effect of Fructus Lycii and Radix Astragali on Synthesis of Inhibin in Sertoli Cells of Rat Testis[J]. Chinese journal of experimental traditional medical formulae, 2007, (1): 58-61.
[1]赵龙坡,徐铮,张文,孙辉臣,汤菲.枸杞、黄芪对大鼠睾丸支持细胞抑制素合成的影响[J].中国实验方剂学杂志,2007(01):58-61. DOI： 10.13422/j.cnki.syfjx.2007.01.023.

ZHAO Long-po, XU Zheng, ZHANG Wen, et al. The Effect of Fructus Lycii and Radix Astragali on Synthesis of Inhibin in Sertoli Cells of Rat Testis[J]. Chinese journal of experimental traditional medical formulae, 2007, (1): 58-61. DOI： 10.13422/j.cnki.syfjx.2007.01.023.

摘要

目的:探讨中药枸杞、黄芪对大鼠睾丸支持细胞功能的作用及机制。方法:对18

2 d的大鼠睾丸支持细胞进行分离培养

用MTT法检测枸杞、黄芪对支持细胞增殖的影响

用免疫组化方法检测枸杞、黄芪对正常状态和过氧化物（H2O2）损伤状态下支持细胞抑制素βB亚基（INHβB）合成的影响。结果:枸杞、黄芪及杞芪合剂对支持细胞的增殖有促进作用;在正常培养状态下

枸杞、黄芪及杞芪合剂对支持细胞INHβB合成具有促进作用;在过氧化物（H2O2）损伤状态下

枸杞、黄芪及杞芪合剂对支持细胞INHβB合成具有保护作用。结论:枸杞、黄芪及杞芪合剂对体外培养的支持细胞抑制素βB亚基合成具有促进和保护作用。

Abstract

Objective:To establish in vitro culture of rat Sertoli cells and to investigate the effects of Chinese herbal medicines to their functions and the mechanism.Methods:Sertoli cells aged 18～22 days were separated and cultured from rat testis;the effect of Fructus Lycii

Radix Astragali and the combined administration of the two medicines on the proliferation of Sertoli cells in vitro were detected by means of MTT assay;the effect of Fructus Lycii

Radix Astragali and the combined administration of the two medicines on the level of INHβB in Sertoli cells in vitro were investigated in both normal environment and peroxide-damaging environment by means of immunohistochemistry.Results:The proliferation of Sertoli cells in vitro was promoted when treated with Fructus Lycii

Radix Astragali and the combined administration of the two medicines;Sertoli cells INHβB was up regulated by Fructus Lycii

Radix Astragali and the combined administration of the two medicines in vitro;Fructus Lycii and Radix Astragali were found to have protective effect on synthesis of INHβB in Sertoli cells with injury induced by peroxide（H

）.Conclusion:The function of promotion and protection were found when Sertoli cells cultured in vitro treated with Fructus Lycii

Radix Astragali and the combined administration of the two medicines.

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