固本化痰通脉方含药血清对大鼠血管平滑肌细胞增殖及细胞周期素D<sub>1</sub>的影响

王平; 程静; 田代志; 邹小娟; 刘松林; 程方平

doi:10.13422/j.cnki.syfjx.2007.09.014

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固本化痰通脉方含药血清对大鼠血管平滑肌细胞增殖及细胞周期素D₁的影响

药理 | 更新时间：2024-09-23

- 固本化痰通脉方含药血清对大鼠血管平滑肌细胞增殖及细胞周期素D₁的影响
- Effect of Guben Huatan Tongmai Recipe on Expression of Cyclin D₁ and the Proliferation of Vascular Smooth Muscle Cells Caused by PDGF-BB Stimulation in Rat
- 中国实验方剂学杂志 2007年第9期页码：22-25
- 作者机构：
  
  1. 湖北中医学院老年医学研究所
  2. 湖北中医学院老年医学研究所湖北武汉430061
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助（30271566）
- DOI：10.13422/j.cnki.syfjx.2007.09.014
  中图分类号： R285.5
- 纸质出版日期：2007
- 稿件说明：
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[1]王平,程静,田代志,邹小娟,刘松林,程方平.固本化痰通脉方含药血清对大鼠血管平滑肌细胞增殖及细胞周期素D_1的影响[J].中国实验方剂学杂志,2007(09):22-25.

WANG Ping~, CHENG Jing, TIAN Dai-zhi, et al. Effect of Guben Huatan Tongmai Recipe on Expression of Cyclin D₁ and the Proliferation of Vascular Smooth Muscle Cells Caused by PDGF-BB Stimulation in Rat[J]. Chinese journal of experimental traditional medical formulae, 2007, (9): 22-25.
[1]王平,程静,田代志,邹小娟,刘松林,程方平.固本化痰通脉方含药血清对大鼠血管平滑肌细胞增殖及细胞周期素D_1的影响[J].中国实验方剂学杂志,2007(09):22-25. DOI： 10.13422/j.cnki.syfjx.2007.09.014.

WANG Ping~, CHENG Jing, TIAN Dai-zhi, et al. Effect of Guben Huatan Tongmai Recipe on Expression of Cyclin D₁ and the Proliferation of Vascular Smooth Muscle Cells Caused by PDGF-BB Stimulation in Rat[J]. Chinese journal of experimental traditional medical formulae, 2007, (9): 22-25. DOI： 10.13422/j.cnki.syfjx.2007.09.014.

摘要

目的:观察固本化痰通脉方对大鼠血管平滑肌细胞生长增殖、细胞周期及细胞周期素D1表达的影响

以探讨其作用机制。方法:血小板源生长因子BB（PDGF-BB）刺激体外培养的大鼠血管平滑肌细胞使其快速增殖生长

加入不同浓度的固本化痰通脉方含药血清作用一定时间后

通过MTT法测定其对血管平滑肌细胞生长的抑制率

流式细胞技术测定细胞周期及细胞周期素D1的表达。结果:固本化痰通脉方能明显抑制PDGF-BB刺激的血管平滑肌细胞增殖

还以浓度依赖方式减少S期细胞、阻滞细胞于G0/G1期

并下调细胞周期素D1的表达。结论:固本化痰通脉方能抑制血管平滑肌细胞的增殖生长

阻滞细胞周期的进行

因而可能具有抗动脉粥样硬化的作用

其作用机理可能与调控血管平滑肌细胞细胞周期素D1的活性有关。

Abstract

Objective:To investigate the effect of Guben Huatan Tongmai Recipe（GHTR） on the proliferation

cell cycle and eyclin D

activity of vascular smooth muscle cells（VSMCs） in rat in order to find out the mechanism of its action.Methods:In this study

cell culture technique was used in vitro.VSMCs stimulated by platelet derived growth factor BB（PDGF-BB） were treated by GHTR in different concentration for a certain period

then the inhibitory ratio of cell proliferation was measured by MTT

cell cycle distribution and cyclin D

activity of the cells were observed by Flow-Cytometry respectively.Results:GHTR could obviously inhibit the proliferation of VSMCs stimulated by PDGF-BB

and it could also decrease S phase cells

increase G

phase cells and down-regulate cyclin D

activity of VSMCs in concentration-dependent fashion

and the results were significant compared with the normal serum plus PDGF-BB-treated.Conclusion:GHTR could obviously inhibit the proliferation of VSMCs and may prevent atherosclerosis

which mechanism may be realized by regulating cyclin D

activity of VSMC.

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固本化痰通脉方含药血清对大鼠血管平滑肌细胞增殖及细胞周期素D1的影响

Effect of Guben Huatan Tongmai Recipe on Expression of Cyclin D1 and the Proliferation of Vascular Smooth Muscle Cells Caused by PDGF-BB Stimulation in Rat

DOI：10.13422/j.cnki.syfjx.2007.09.014

摘要

Abstract

关键词

Keywords

references

固本化痰通脉方含药血清对大鼠血管平滑肌细胞增殖及细胞周期素D₁的影响

Effect of Guben Huatan Tongmai Recipe on Expression of Cyclin D₁ and the Proliferation of Vascular Smooth Muscle Cells Caused by PDGF-BB Stimulation in Rat