Objective:To study the effection of Tangnai-Kang conction for insulin resistance and expression of Phosphatidylinositol 3 Kinase、Glucose Transport 4 messenger ribonucleic acid（mRNA）.Methods:Specific pathogen free（SPF） KKAy mice 40 were divided randomly according to plasma glucose level into Models

TNK-low dosage

TNK-high dosage

Pioglitazone and normal C57BL/6J mice（normal control group）.60 days later

all animals were tested Fasting plasma glucose（PFG）

Fasting insulin level for caculating Insulin Sensitivity Index（ISI）

Total Cholesterol（TC）

Total triacylglycerol（TG）

High-density Lipoprotein Cholesterol（HDL-C）

Low-density Lipoprotein Cholesterol（LDL-C）

Floating fatty acid（FFA）

Tumor necrosis factor-α（TNF-α） and pathological examination for the islet.The muscle and adipose expressio of PI-3K and GluT4 mRNA by reverse transcriptase PCR（RT-PCR） were determined.Results:Compared with Model groups

FPG、TNF-α levels were decreased significantly for TNK-high dosage（P<0.05）

the expression of PI-3K and GluT4 mRNA were all up-regulated（P<0.05 or P<0.01）

the recipe could protect β-cells in some degree and postponting β-cells failure in islet

displayed decreasing tendency to plasma lipocy and FFA levels.Conclusions:One of the mechanism of Tangnai-Kang improving insulin resistance might be up-regulated the expression of PI-3K mRNA in post-receptor of insulin signal-transduction roads

thereby increased GluT4 translocation.