HPLC法测定痛经膏贴中黄芩苷和汉黄芩素含量研究

韩建伟; 刘颖; 魏元锋; 张晓燕

doi:10.13422/j.cnki.syfjx.2008.08.008

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HPLC法测定痛经膏贴中黄芩苷和汉黄芩素含量研究

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- HPLC法测定痛经膏贴中黄芩苷和汉黄芩素含量研究
- Determination of Baicalin and Wogonin in Tongjing Patch by HPLC
- 中国实验方剂学杂志 2008年第8期页码：8-10
- 作者机构：
  
  1. 湖北中医学院中药资源与中药复方省部共建教育部重点实验室
  2. 健民药业集团
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2008.08.008
  中图分类号： R286
- 纸质出版日期：2008
- 稿件说明：
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[1]韩建伟,刘颖,魏元锋,张晓燕.HPLC法测定痛经膏贴中黄芩苷和汉黄芩素含量研究[J].中国实验方剂学杂志,2008(08):8-10.

HAN Jian-wei1, LIU Ying2, WEI Yuan-feng1, et al. Determination of Baicalin and Wogonin in Tongjing Patch by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2008, (8): 8-10.
[1]韩建伟,刘颖,魏元锋,张晓燕.HPLC法测定痛经膏贴中黄芩苷和汉黄芩素含量研究[J].中国实验方剂学杂志,2008(08):8-10. DOI： 10.13422/j.cnki.syfjx.2008.08.008.

HAN Jian-wei1, LIU Ying2, WEI Yuan-feng1, et al. Determination of Baicalin and Wogonin in Tongjing Patch by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2008, (8): 8-10. DOI： 10.13422/j.cnki.syfjx.2008.08.008.

摘要

目的:探索HPLC测定痛经膏贴中黄芩苷和汉黄芩素含量的方法。方法:Zorbax SB-C18（4.6mm×250mm

5μm）色谱柱;流动相:甲醇-水-磷酸（58:42:0.05）;流速:1.0mL.min-1;检测波长:278nm。结果:黄芩苷的线性范围为（0.44～2.20）μg（r=0.9997）

平均回收率为98.64%

RSD=1.17%;汉黄芩素的线性范围为（0.0128～0.2048）μg（r=0.9999）

平均回收率为98.96%

RSD=2.08%;3批样品中黄芩苷和汉黄芩素的含量分别为7.33%、6.74%、7.29%和0.71%、0.65%、0.63%。结论:测定方法简便、准确

可同时测定痛经膏贴中黄芩苷和汉黄芩素含量。

Abstract

Objective:To develop an HPLC method to determine the contents of both baicalin and wogonin in Tongjing Patch.MethodA Zorbax SB-C18 column（4.6×250 mm

5 μm）was used.The mobile phase was composed of methanol-water-acetic acid（58∶42∶0.05） and the flow rate was 1.0 ml·min-1.UV detection wave length was set at 278 nm.Results:The linear range of baicalin was（0.44～2.20）μg（r= 0.999 7）and the average recovery was 98.64% and the RSD was 1.17%.The linear range of wogonin was（0.012 8 ～ 0.204 8）μg（r= 0.999 9）and the average recovery was 98.96% and the RSD was 2.08%.In three batches of the patches

the contents of baicalin were 7.33%

6.74% and 7.29% and that of wogonin were 0.71%

0.65% and 0.63%.Conclusion:This method is simple and accurate and can be used for the determination of the contents of both baicalin and wogonin in Tongjing Patch.

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